地黄饮子对脑缺血后大鼠海马BDNF的影响

目的研究地黄饮子对大鼠持续性脑缺血后海马内脑源性神经营养因子(BDNF)的影响.方法用线栓法建立大鼠脑缺血模型,以免疫组化技术显示地黄饮子在缺血后对海马区BDNF的影响.结果在大鼠脑缺血区有BDNF阳性神经元表达,缺血组和给药组海马区神经元随缺血时间的延长,存活细胞数目均逐渐减少,而给药组存活的神经细胞数目较缺血组多.结论地黄饮子可通过促进BDNF表达,抑制细胞死亡,促进脑缺血后海马神经元的存活和修复.

Effects of Dihuangyinzi Decoction on Brain-Derived Neurotrophic Factor in the Hippocampus of the Rat with Persistent Ischemia

Objective To study the effects of Dihuangyinzi Decoction (DD) on brain-derived neurotrophic factor (BDNF) in the hippocampus of the rat with persistent ischemia. Method The rat model of ischemia was established by ligation. The effects of DD on BDNF in the hippocampus of the rat model of ischemia were observed by using immunohistochemical techniques.Results It was found that there was the expression of BDNF-positive neurons in the ischemic region of the rat brain;and although the survived neurons gradually reduced along with the prolongation of the ischemia time in the hippocampal regions of the rats in both the ischemia group and DD-administration group,the survived neurons in the DD-administration group were more than those in the ischemia group. Conclusion DD can inhibit the death of the neurons and promote the survival and repair of the neurons in the hippocampus through promoting the expression of BDNF in the cerebrally ischemic rat