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Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells |
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| Authors: | Wang Yun-Shan Ma Xiao-Li Qi Tong-Gang Liu Xiang-Dong Meng Yue-Sheng Guan Guang-Ju |
| Institution: | 1. Central Laboratory, Jinan Central Hospital, Clinical Medical College of Shandong University, Jinan 250013, Shandong Province, China 2. Laboratory of Clinical Molecular Biology, The Second Hospital of Shandong University, Jinan 250015, Shandong Province, China 3. Shandong Provincial Hospital, Jinan 250020,Shandong Province, China |
| Abstract: | AIM: To study the function of alpha-fetoprotein (AFP) in SMMC-7721 hepatoma cells. METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescence activated cell sorter (FACS). RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis. CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis. |
| Keywords: | siRNAs AFP SMMC-7721 cell Proliferation Apoptosis |
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