巨噬细胞和淋巴细胞及增殖细胞核抗原在鼻息肉组织中的表达及病理学意义

目的　研究巨噬细胞 (CD68)、T细胞 (CD45RO)、B细胞 (CD2 0 )和增殖核细胞核抗原(proliferatingcellnuclearantigen ,PCNA)在鼻息肉组织中的表达。方法　应用免疫组化链霉菌抗生物素蛋白过氧化酶 (strept avidinoxidase,SP)法对 50例鼻息肉分别做CD2 0、CD45RO、CD68、PCNA的免疫组化染色 ,结合常规HE染色切片进行分析。结果　①CD68+ 细胞在嗜酸性粒细胞性鼻息肉较嗜中性粒细胞性鼻息肉表达率高 ,差异具有显著性意义 (P <0 0 5) ;②CD45RO、CD2 0在鼻息肉均有阳性表达 ,CD45RO与CD2 0呈负相关 (P =0 0 5) ;③CD68阳性细胞与嗜酸性粒细胞浸润及鼻息肉上皮PCNA阳性表达有相关性 (P <0 0 5)。鼻息肉上皮的PCNA阳性表达和成纤维细胞PCNA阳性表达有相关性 ,(P <0 0 5)。结论　鼻息肉的形成与炎性细胞浸润密切相关。鼻黏膜局部的细胞免疫与体液免疫异常导致上皮细胞、成纤维细胞增殖、腺体增生是鼻息肉发生的基础 ,CD68+ 细胞可能是鼻息肉中的炎性干细胞

Expression of CD68 CD45RO CD20 and proliferating cell nuclear antigen in nasal polyps

Objective To study the cellular expression of CD45RO,CD20,CD68 and proliferating cell nuclear antigen (PCNA )in nasal polyps Methods Nasal polyp tissues from 50 patients were evaluated for cellular expression of CD45RO ,CD20,CD68 and PCNA using immunohistochemistry SP by counting the average number in 5 chosen high power fields, Histopathological observations were combined with HE Analyses were performed on SPSS10 0 Results CD68 + cells were expressed more in nasal polyps dominated by eosinophils than by neutrophils( P <0 05) There was no difference between CD45RO and CD20,but both of them had negative correlation( P =0 05) Significant correlation was found between CD68 + cells and eosinophils or PCNA positive cells on epithelium PCNA positive cells on epithelium had significant correlation on fibroblast ( P <0 05) Conclusion Inflammatory cell infiltration(eosinophilia CD45RO?CD20?CD68 )and cell proliferating in epithelium cells ,glandular cell and fibroblast are strongly correlated with formation of nasal polyps The nasal polyps are not only characteristic of eosinophilia but also by lymphocytes dominated by CD45RO and CD68 positive cells CD68 may be stem cell of nasal polyp