复合骨形成蛋白骨修复材料的生物相容性研究

目的 :近年来脱钙骨基质颗粒复合骨水泥已用于修复微波诱导高温原位灭活骨肿瘤性骨缺损 ,在此基础上再复合骨形成蛋白可提高其成骨诱导活性。为推广临床应用 ,本文拟对其生物相容性进行研究。方法 :制备脱钙骨基质颗粒 ,提取牛骨形成蛋白 ,与骨水泥按不同质量比复合后植入小白鼠腹腔内行急性、亚急性毒性试验 ,植入小白鼠后腿股部肌肉内行肌肉刺激试验 ,体外行溶血、凝血试验和骨内埋入试验。结果 :急性毒性试验动物无死亡 ,亚急性毒性试验术前、术后动物体重、红细胞、白细胞和血红蛋白无统计学差别 (t检验 ,P >0 .0 5 ) ,对肌肉无刺激 ,体外对溶血、凝血功能无影响 ,植入骨内无不良反应 ,6个月即可与宿主骨形成“生物铆定”。结论 :该复合材料生物相容性好 ,易塑形 ,具有很强的成骨诱导活性 ,能满足修复不同部位、不同形状骨缺损的需要

Study on Biocompatibility of Material Combined with Bone Morphogenetic Protein

Objective: The decalcified bone matrix(DBM) combined with bone cement (BC) had been used for the repair of bone defect caused by the bone tumors treated with microwave induced hyperthermia. However the bone inductive activity could be improved when the DBM BC composite was further combined with bovine bone morphogenetic protein (bBMP). This study was to investigate the biocompatibility of the material combined with bBMP and to further offer the use for clinic. Methods: The alien canine DBM was prepared. The bBMP was extracted and mixed with DBM with the weight proportion of 1:25. Then the bBMP DBM composite was combined with BC with different weight proportion and the composite material was tested by acute and subacute toxicity test, muscle stimulation test, hemolysis and coagulation test and was examined by being implanted in the canine femur. Results: No animals died in the acute toxicity test and the body weight, red blood cells, white blood cells and hemoglobin were not effected in the subacute test. The composite material did not effect the function of hemolysis and coagulation and could be merged with the normal bone at the 6th month postoperatively. Conclusions: This composite material has excellent biocompatibility and bone inductive activity. It is favorable for the repair of bone defects with different shape and different position because it can be also modeled easily.