维生素E镇痛作用及机制的研究

 目的：研究维生素E(Vit E)的镇痛作用及机制。方法：在小鼠热板和扭体反应模型上，观察用药后舔足潜伏期和扭体数改变，丙二醛(MDA)用巴比妥酸法测定，一氧化氮含量用Green法测定。结果：100，200mg·kg^-1VitEim可显著延长小鼠舔足潜伏期和减少小鼠扭体数，并且100mg·kg^-1VitE的镇痛作用可被EGTA加强，CaCl₂所拮抗，但维拉帕米可部分逆转CaCl₂对VitE镇痛的拮抗；VitE产生镇痛时，也可显著抑制小鼠脑组织中MDA和NO含量增高，并可被EGTA增强，CaCl₂所减弱。结论：上述结果表明Vit E有显著的镇痛作用，其作用与减少脑内NO和MDA生成有关，并被Ca²⁺所影响。

The analgesic effect of vitamine E and its mechanism

OBJECTIVE: To study the analgesic effect of vitamine E and its mechanism. METHODS: On the hot plate and writhing test in mice, the changes of the latencies of licking paws and the writhing numbers were observed after drug administration. The content of malondialdehyde (MDA) was measured with thiobarbituric acid method. Green method was used for the determination of nitric oxide (NO) content. RESULTS: 100, 200 mg·kg^-1 vitamin E could significantly prolong the latencies of licking paws and reduce the writhing numbers. The analgesic effect of vitamin E could be antagonized by CaCl₂ and enhanced by EGTA. Verapamil partially overturned the antagonism of CaCl₂ on the analgesic effect of vitamin E. The increase of NO and MDA contents in the cerebrum was inhibited by vitamin E when analgesic effect of vitamin appeared. This inhibitory effect could also be weakened by CaCl₂ and enhanced by EGTA. CONCLUSION: Vitamin E has significant analgesic effect and its effect related to the reduction of MDA and NO in brain and was influenced by calcium ion.