半边旗提取物5F对人鼻咽癌CNE-2Z细胞生长的抑制作用

目的研究半边旗提取物5F对人鼻咽癌CNE-2Z细胞的抑制作用及可能机制。方法以台盼蓝活细胞计数法测定生长曲线;用MTT比色法检测给药后鼻咽癌细胞的增殖抑制情况;采用FCM检测5F对细胞周期和凋亡的影响;用PI/Hoechst33258荧光双染法和DNA ladder检测凋亡。结果 5F作用后,生长曲线测定和MTT比色法实验结果显示,5F对CNE-2Z细胞的增殖和生长有显著的抑制作用并呈明显的时间-剂量依赖关系;流式细胞仪、DNA ladder和荧光双染法结果提示5F可诱导CNE-2Z细胞凋亡。结论半边旗提取物5F能抑制CNE-2Z细胞的增殖效应,其机制可能与诱导细胞凋亡和引起细胞周期阻滞于G2/M期有关。

Inhibitory Effects of 5F from Pteris semipinnata L.on Proliferation of Human Nasopharyngeal Carcinoma CNE- 2Z Cells

Objective To study the inhibitory effect and its mechanism of 5F from Pteris semipinnata L.on human nasopharyngeal carcinoma CNE-2Z cells. Methods The growth curve was drawn by means of counting cell number.MTT assay was adopted to determine the inhibition rate on CNE-2Z cells.The cell apoptosis was detected by flow cytomertic analysis,DNA ladder and Hoechst3358/PI fluorescent staining. Results The result of growth curve and MTT showed that the 5F had a remarkable inhibition on the proliferation and growth of CNE-2Z cells,in time and does-dependent manner.FCM,DNA ladder and fluorescent staining could detect the cell apoptosis. Conclusion 5F can inhibit the proliferation of CNE-2Z cells and the possible mechanism may be related to inducing the cell apoptosis and arresting the cell cycle in G2/M phase.