| 清开灵片中胆酸及栀子苷含量的高效液相色谱测定 |
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| 引用本文： | 笔雪艳,刘晓凤,胡畔,张清波.清开灵片中胆酸及栀子苷含量的高效液相色谱测定[J].时珍国医国药,2012,23(3):663-665. |
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| 作者姓名： | 笔雪艳 刘晓凤 胡畔 张清波 |
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| 作者单位： | 1. 黑龙江省药品检验所,黑龙江哈尔滨,150001
2. 黑龙江中医药大学,黑龙江哈尔滨,150040 |
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| 基金项目： | 《中国药典》2010版Ⅰ部标准研究课题 |
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| 摘 要： | 目的建立清开灵片中胆酸及栀子苷的含量测定方法。方法采用HPLC,分别以蒸发光散射和紫外检测器为检测手段,测定制剂中胆酸及栀子苷的含量。结果清开灵片中胆酸在0.588 6～3.924 0μg之间呈良好的线性关系(r=0.999 3),平均回收率为100.22%,RSD4.1%;栀子苷在0.030 12～0.903 6μg之间呈良好的线性关系(r=1),平均回收率为103.8%,RSD为2.0%。结论该方法简便、准确、分离效果好,可用于清开灵片的质量评价。
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| 关 键 词： | 清开灵片 胆酸 栀子苷 高效液相色谱 |
Determination of Cholic Acid and Geniposide in Qingkailing Tablets by HPLC |
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| BI Xue-yan
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LIU Xiao-feng
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HU Pan
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ZHANG Qing-bo.Determination of Cholic Acid and Geniposide in Qingkailing Tablets by HPLC[J].Lishizhen Medicine and Materia Medica Research,2012,23(3):663-665. |
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| Authors: | BI Xue-yan LIU Xiao-feng HU Pan ZHANG Qing-bo |
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| Institution: | 1(1.Heilongjiang Institute for Food and Drug Control,Harbin 150001,China;2.Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China) |
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| Abstract: | Objective To establish the determination method of cholic acid and geniposide in Qingkailing tablets by HPLC. Methods HPLC with ELSD and UV detector was used to determine the contents of cholic acid and geniposide. Results Cholic acid showed good linear relationship(r=0.999 3) within the range of 0.588 6～3.924 0 μg,and the average recovery rate was 100.22 %(RSD= 4.1 %,n=6);Geniposide showed good linear relationship(r=1),0.030 12～0.903 6 μg,and the average recovery rate was 103.8 %(RSD=2.0 %,n=6). Conclusion This method is simple,accurate,and separating effect is good.It can be used for quality control of Qingkailing tablets. |
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| Keywords: | Qingkailing tablets Cholic acid Geniposide HPLC |
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