| 苍术抗流感病毒有效成分的筛选 |
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| 引用本文： | 石书江,秦臻,孔松芝,赖小平,苏子仁,曾惠芳. 苍术抗流感病毒有效成分的筛选[J]. 时珍国医国药, 2012, 23(3): 565-566 |
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| 作者姓名： | 石书江 秦臻 孔松芝 赖小平 苏子仁 曾惠芳 |
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| 作者单位： | 1. 广州中医药大学第一附属医院,广东广州,510405
2. 广州中医药大学中药学院,广东广州,510006 |
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| 基金项目： | 国家自然科学基金,国家科技重大专项 |
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| 摘 要： | 目的从苍术中筛选抗流感病毒的有效成分。方法采用水提、超临界二氧化碳流体(SFE-CO2)提取,并利用硅胶柱层析法进行成分分离,得到苍术的各种提取物和有效成分单体,再用流感病毒甲型H3N2、H5N1(禽流感病毒)和乙型流感病毒进行狗肾传代细胞株(MDCK)组织培养筛选。结果含苍术酮的苍术SFE-CO2提取物及苍术酮,对流感病毒有杀灭作用;而苍术素、苍术的水提液对流感病毒无抑制作用。结论苍术酮是苍术中抗流感病毒的有效成分。
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| 关 键 词： | 苍术 苍术酮 流感病毒 筛选 |
Effective Component Selection of Atraotydin Extract against Influenza Virus |
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| SHI Shu-jiang , QIN Zhen , KONG Song-zhi , LAI Xiao-ping , SU Zi-ren , ZENG Hui-fang. Effective Component Selection of Atraotydin Extract against Influenza Virus[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(3): 565-566 |
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| Authors: | SHI Shu-jiang QIN Zhen KONG Song-zhi LAI Xiao-ping SU Zi-ren ZENG Hui-fang |
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| Affiliation: | 1 (1.The No.1 Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;2.Guangzhou University of Chinese Medicine,School of Chinese Materia Medica,Guangzhou 510006,China) |
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| Abstract: | Objective To screen the active components against anti influenza virus in Atraotydin. Methods The extracts were separated by supercritical CO2 fluid extraction(SFE-CO2),aqueous extraction and silica column chromatography.Madin-Darby Canine Kidney(MDCK) cell tissue culture was used to screen the effective ingredient with fluenza A virus and influenza B virus. Results Atractylon and the SFE-CO2 Atracylodes Lancea Rhizoma could kill influenza virus;Atractylodin and the water extract from Rhizoma Atractylodis had no effect on influenza virus. Conclusion Atractylon is effective ingredient for anti influenza virus in Atraotydin. |
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| Keywords: | Atraotydin Atractylon Influenza virus Screening experiment |
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