Preliminary study of retroviral mediated transfer of multidrug resistance gene into CD34~ cells originated from human bone marrow

To elucidate the feasibility of retroviral mediatedtransfer of multidrug resistance gene (mdrl) intohematopoietic stem/progenitor cells, the CD34~ cellswere isolated from bone marrow by using a high-gradient.magnetic cell sorting system, and the efficiency ofretroviral mediated gene transfer was studied. Materials