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| 乳腺癌和非癌组织中DLC1基因及蛋白表达与Ki-67的关系 | |
| 引用本文： | 刘宇飞,张兆祥,淡汉进,陈路,黄益玲,高艳,韩林. 乳腺癌和非癌组织中DLC1基因及蛋白表达与Ki-67的关系[J]. 临床与实验病理学杂志, 2010, 26(1) |
| 作者姓名： | 刘宇飞 张兆祥 淡汉进 陈路 黄益玲 高艳 韩林 |
| 作者单位： | 1. 三峡大学医学院病理学教研室,宜昌,443002 2. 湖北省枝江市人民医院病理科,枝江,443200 3. 三峡大学第一临床医学院病理科,宜昌,443003 |
| 摘 要： | 目的探讨乳腺癌和非癌组织中DLC1表达及其与Ki-67的相互关系。方法应用原位杂交技术和免疫组织化学En-Vision两步法,检测52例乳腺浸润性导管癌和42例非癌组织(包括癌旁乳腺组织20例和乳腺纤维腺瘤22例)中DLC1-mR-NA、DLC1和Ki-67的表达。结果DLC1-mRNA和蛋白在乳腺癌中的阳性率(50%和57.7%)低于非癌乳腺组织(90.5%和92.9%),差异有统计学意义(χ2=17.518和10.729,P0.01)。DLC1-mRNA与蛋白的表达呈正相关(rs=0.379,P0.01)。Ki-67在乳腺癌中的阳性率为61.5%,而在非癌组织中均呈阴性表达,两者差异有显著统计学意义(χ2=39.186,P0.01)。乳腺癌中DLC1与Ki-67的表达呈负相关(rs=-0.507,P0.01)。结论DLC1-mRNA和蛋白在乳腺癌中呈低表达或缺失,提示其与乳腺癌的发生、发展有关,DLC1有抑制乳腺癌细胞增殖的功能,可作为乳腺癌新的分子标记物。联合检测DLC1和Ki-67在乳腺癌中的表达,有望成为估价乳腺癌生物学行为的参考指标。 |
| 关 键 词： | 乳腺肿瘤 DLC1 Ki-67 免疫组织化学 原位杂交 |
Expression of DLC1 mRNA and protein in breast carcinoma and non-cancerous breast tissues and its relationship with Ki-67 |
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| LIU Yu-fei,ZHANG Zhao-xiang,DAN Han-jin,CHEN Lu,HUANG Yi-ling,GAO Yan,HAN Lin. Expression of DLC1 mRNA and protein in breast carcinoma and non-cancerous breast tissues and its relationship with Ki-67[J]. Chinese Journal of Clinical and Experimental Pathology, 2010, 26(1) | |
| Authors: | LIU Yu-fei ZHANG Zhao-xiang DAN Han-jin CHEN Lu HUANG Yi-ling GAO Yan HAN Lin |
| Affiliation: | 1Department of Pathology/a>;China Three-Gorges University Medical College/a>;Yichang 443002/a>;China/a>;2Department of Pathology/a>;Zhijiang People's Hospital/a>;Zhijiang 443200/a>;3Department of Pathology/a>;the First College of Clinical Medical Science/a>;China Three-Gorges University/a>;Yichang 443003/a>;China |
| Abstract: | Purpose To investigate the expression of DLC1 and its relationship with Ki-67 in cancerous and non-cancerous tissues of the breast.Methods In situ hybridization and immunohistochemiscal EnVision method were used to detect the expression of DLC1 mRNA and protein and Ki-67 in 52 invasive breast ductal carcinomas and 42 non-cancerous mammary tissues,including 22 mammary fibroadenomas and 20 paracancerous tissues.Results The positive rates of DLC1 mRNA and protein expression in the breast carcinomas (50% and 57... |
| Keywords: | breast neoplasms DLC1 Ki-67 immunohistochemisty in situ hybridization |
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