C57BL/6小鼠上皮性卵巢癌模型的建立及其生物学特性

目的在免疫功能正常的C57BL/6小鼠体内建立上皮性卵巢癌腹腔转移瘤模型及皮下瘤模型,为卵巢癌的诊断、治疗及预防的相关研究提供基础。方法体外培养近交系C57BL/6小鼠卵巢上皮低分化腺癌细胞株ID-8,将对数生长期的ID-8细胞按1×10^7、5×10^6、1×10^6和1×10^5个细胞/只的剂量,分别接种于6～8周雌性SPF级C57BL/6小鼠腹腔及左侧肩部皮下,共8组,每组6只。观察腹腔瘤及皮下瘤的成瘤时间、成瘤率、腹水、腹腔肿瘤转移情况及小鼠生存期;处死小鼠时留取主要脏器、腹腔肿瘤及皮下肿瘤标本,行病理学检查。另外6只小鼠接种5×10^6个ID-8细胞,分别在4、8、16周后处死进行系统解剖,做常规病理学检查。结果将不同数量的ID-8细胞接种C57BL/6小鼠腹腔及皮下后,成瘤率均为100%,腹腔瘤模型组：腹腔注射1×10^5,1×10^6,5×10^6,1×10^7个ID-8细胞,平均生存时间分别为（141±6.7）d、（122.8±4.5）d、（83.4±7.2）d和（74.4±4.5）d,随着肿瘤细胞接种负荷增加,动物生存期明显缩短（P〈0.05）。皮下瘤组：1×10^7细胞组和5×10^6细胞组,1周左右成瘤;1×10^6细胞组,3周左右成瘤;1×10^5细胞组,6周左右成瘤。随着肿瘤接种负荷的增加,肿瘤直径和体积明显增大（P〈0.05）。结论 C57BL/6小鼠腹腔瘤模型类似人类Ⅲ、Ⅳ期卵巢上皮癌患者的临床特点。皮下瘤模型更易于观察免疫治疗或药物治疗的疗效。在免疫功能正常的C57BL/6小鼠建立的ID-8细胞卵巢癌肿瘤模型,是适合于卵巢癌分子和免疫治疗研究的模型。

C57BL/6 mouse model of epithelial ovarian cancer and its biological characteristics

Objective To establish epithelial ovarian cancer of peritoneal metastasis model and subcutaneous tumor model in immunocompetent C57BL/6mice , thus to provide basis of diagnosis , treatment and prevention-related basic research for ovarian cancer.Methods Cultured inbred C57BL/6mice poorly differentiated ovarian adenocarcinoma cell line ID-8in vitro.SPF grade C57BL/6female mice (6～8weeks) were inoculated with logarithmic phase of ID-8cells with 1×10~7 , 5×10~6 , 1×10~6 and l×l05cells / dose intraperitoneally and subcutaneously into the flank.They were invided into 8groups with 6in each.Tumor formation rate , ascites , metastasis and survival rate were recorded.An additional set of 6animals were injected intraperitoneally (5×10~6 ID-8cells) and then sacrificed at different time intervals for necropsy and pathologic analysis.The remaining animals were sacrificed and examined just before they died.Results The tumor formation rate was 100%.In peritoneal tumor model group , the average survival time was (141±6.7) d ,(122.8±4.5) d ,(83.4±7.2) d ,(74.4±4.5) d in 1×10 ~5 , 1×10~6 , 5 ×10~6 and l×10~7 ID-8cells group , with the tumor cell load increased , the survival time was significantly shorter (P<0.05) .In subcutaneous tumor group , nodule formation time was about 1week in l×10~7 cells group ; 1week in 5×10~6 cells group ; 3weeks in 1×10~6 cells group and 6weeks in 1×10~5 cells group.As the tumor cells inoculation load increased , the diameter and volume of tumor increased significantly (P<0.05) .Conclusions C57BL/6 mice intraperitoneal ovarian cancer model is similar to human epithelial ovarian cancer stageⅢ and Ⅳ.The immune or drug therapy efficacy is easier observed in subcutaneous tumor model. Establishing the ID-8cells ovarian cancer tumor model in immunocompetent C57BL/6 mice is helpful for clinical study of the treatment of ovarian cancer and immunological study of molecular models.