牛磺酸对血管紧张肽Ⅱ诱导的大鼠心肌成纤维细胞增殖的抑制作用

目的：研究牛磺酸对血管紧张肽Ⅱ(AngⅡ)诱导的新生大鼠心肌成纤维细胞增殖和胶原合成的抑制及对转化生长因子β_1(TGF-β_1)蛋白表达的下调作用,以探讨其对抗AngⅡ诱导的心肌纤维化的作用。方法：在AngⅡ诱导心肌成纤维细胞增殖前后加不同浓度的牛磺酸,并采用羟脯氨酸法、MTT比色法分别测定胶原量和细胞增殖情况、SDS-PAGE电泳测胶原Ⅰ/Ⅲ型比值、ELISA法测定TGF-β_1蛋白的含量。结果：(1) AngⅡ有促进心肌成纤维细胞增殖及胶原合成的作用,其浓度在1×10~(-7)mmol·L~(-1)时达最大效应；(2)牛磺酸能抑制由AngⅡ诱导的Ⅰ/Ⅲ型胶原比值增高和TGF-β_1蛋白含量的增加,其中100,200 mmol·L~(-1)的牛磺酸可抑制AngⅡ诱导的细胞增殖和胶原合成。结论：牛磺酸可用于抑制由AngⅡ导致的心肌纤维化。

Inhibition of taurine on angiotensin Ⅱ- induced synthesis of collagen and pro liferation of cardiac fibroblasts in rats

AIM:To investigate the inhibition of taurine against angiotensinⅡ(AngⅡ)-induced collagen synthesis,cardiac fibroblastic(Fbs)proliferation and the downward regulation of expression of transforming growth factorβ_1(TGF-β_1);and explore the protective action of taurine on AngⅡ-induced myocardial fibrosis. METHODS:We added taurine in different dosages before and after AngⅡinducing Fbs.Hydroxyproline mea- surment and MTT chromatography were carried out for detecting collagen quantification and cellular proliferation. SDS-PAGE electrophoresis determined the ratio of collagenⅠ/Ⅲand ELISA method detected the quantity of TGF-β_1.RESULTS:Taurine blocked the increase of taurine ratio of collagenⅠ/Ⅲand also inhibited elevation of TGF-β_1;with concentation at 100 mmol·L~(-1) and 200 mmol·L~(-1) taurine also attenuated AngⅡ-induced collagen synthesis and cardiac Fbs proliferation.CONCLUSION:Taurine could provide protective action against AngⅡ- induced myocardial fibrosis.