大鼠失血性休克及再灌注后小动脉的形态学改变

目的:探讨失血性休克和再灌注对大鼠小动脉形态结构的影响.方法:70只大鼠随机分为正常对照组、缺血45 min组和缺血再灌注1、3、6、12、24 h组,达到各预定时间点后,迅速取大鼠的脑、肾、四肢腓肠肌制作切片,采用光镜和透射电镜观察各组大鼠各组织小动脉内皮、平滑肌细胞的形态学改变.结果:缺血45 min组大鼠小动脉轻微损伤,小动脉内皮细胞肿胀,异染色质增多,染色质边积,平滑肌细胞轻微水肿,线粒体肿胀.再灌注后各组损伤加重,小动脉部分内皮坏死、脱落,内皮细胞胞浆内出现空泡,血管平滑肌细胞严重水肿,平滑肌细胞向内膜迁移,线粒体肿胀,空泡样变,核周隙增宽,胞质内可见髓样小体.以再灌注3 h损伤最显著,与其它组织相比,脑组织小动脉在缺血及再灌注后不同时点的损伤较轻,再灌注6 h后逐渐恢复.结论:失血性休克和再灌注可引起大鼠小动脉内皮、平滑肌细胞损伤.

Effects of hemorrhagic shock and reperfusion on the morphological changes of small artery in rats

Objective: To observe the morphological changes of small artery after hemorrhagic shock and rerfusion in rats. Methods: Speciman of tissues were taken in the ischemia 45 minutes and reperfusion for 1, 3, 6, 12, 24 hours. By light microscope and electron microscope, observe the morphological changes of small artery. Results: Light microscope and electron microscope: The injury degree of small artery in 45 min ischemia group was slight, the injury degree of reperfusion was more severe than ischemia group. The most severe cellular damage was found in 3 hour after reperfusion. Compared with other tissues, the injury degree of small artery in brain was more slightly. Conclusion: Hemorrhagic shock and reperfusion can induce morphological changes of small artery. Hemorrhagic shock and reperfusion can induce ischemia/reperfusion damage of small artery.