脑损伤后大鼠脑干胶质细胞源性神经营养因子及其受体的表达

目的探讨创伤性脑损伤对大鼠脑干胶质细胞源性神经营养因子(GDNF)及其受体(GFRα-1、Ret)表达的影响。方法采用Marmarou's大鼠落体打击损伤模型,将55只大鼠随机分为正常对照组、手术对照组及损伤后1h组、2h组、4h组、8h组、12h组、24h组、48h组、72h组和5d组,每组5只。制备组织芯片,采用免疫组化法检测脑干GDNF、GFRα-1、Ret的表达情况。结果正常对照组、手术对照组脑干中可见到GDNF及其受体低水平表达,损伤后2h脑干GDNF的表达达到高峰,损伤后72h降至正常水平;GFRα-1和Ret的表达在损伤后4h达到高峰,于伤后48h降至正常。结论大鼠创伤性脑损伤后脑干GDNF及其受体在早期即明显表达,两者在脑损伤后表达的时序性变化特点基本一致。

Expressions of Glial Cell Line-derived Neurotrophic Factor and Its Receptors in Brain Stem of Rats after Brain Injury

Objective To investigate the effect of traumatic brain injury on the expressions of glial cell line-derived neurotrophic factor (GDNF) and its receptors in brain stem of rats.Methods 55 male Sprague-Dawley rats were divided into the normal control group, sham surgery group and injury groups. The rats of injury groups were subjected to Marmarou's closed traumatic brain injury and then were subdivided into 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h and 5 d groups according to the time elapsed after injury. The expressions of GDNF and its receptors (GFRα-1 and Ret) were tested with immunohistochemistry.Results Mild expressions of GDNF and its receptors were observed in brain stem of rats in the normal control group and sham surgery group. The number of GDNF positive neurons reached the peak level at 2 h in brain stem after injury, and that of GFRα-1 and Ret positive neurons reached the peak level at 4 h after injury.Conclusion The expressions of GDNF and its receptors increase significantly at the early time in brain stem of rats after injury. The similar temporal patterns of expressions of GDNF and its receptors are observed in brain stem after brain injury.