| 聚乙二醇修饰羊生长激素的初步研究 |
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| 引用本文： | 聂磊,麦荫乔,赵秋宇,王树歧,程玉华. 聚乙二醇修饰羊生长激素的初步研究[J]. 中山大学学报(医学科学版), 1992, 0(2) |
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| 作者姓名： | 聂磊 麦荫乔 赵秋宇 王树歧 程玉华 |
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| 作者单位： | 中山医科大学生化教研室，中山医科大学生化教研室，吉林大学酶工程实验室，吉林大学酶工程实验室，吉林大学酶工程实验室 白求恩医科大学 |
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| 摘 要： | 使用非抗原性聚乙二醇(PEG)修饰羊生长激素(OVine growth hormone,oGH),分别获得氨基修饰率为35％和86％的PEG-oGH。氨基修饰率为35％的PEG-oGH仍保留了与天然oGH抗血清的反应性,但修饰率为86％的PEG-oGH则丧失了。荧光光谱分析表明,与天然oGH比较,修饰率为35％的PEG-OGH仅其荧光强度有所减弱,光谱图并未改变,但修饰率为86％的PEG-oGH则发生了明显的图谱变化。体内结合试验表明,两种修饰oGH与肝受体结合之能力均有明显降低。
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| 关 键 词： | 羊生长激素 聚乙二醇修饰 抗原性与生物活性 荧光光谱 体内结合试验 |
PRELIMINARY STUDY ON MODIFICATION OF OVINE GROWTH HORMONE WITH POLYETHYLENE GLYCOL |
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| Nie Lei Mai Yinqiao. PRELIMINARY STUDY ON MODIFICATION OF OVINE GROWTH HORMONE WITH POLYETHYLENE GLYCOL[J]. Journal of Sun Yatsen University(Medical Sciences), 1992, 0(2) |
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| Authors: | Nie Lei Mai Yinqiao |
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| Abstract: | Polyethylene glycol (PEG), a non-antigenic polymer, has been used to modify ovine growth hormone (oGH), and two modified oGH, whose free amino groups have been modified by 35% and 86% respectively were produced under the chosen condition. The 35% modified PEG-oGH reacted slightly with antibodies raised against native oGH, but the 86% modified portion could not, ie its antigenicity was lost. Fluorescent spectrum analysis showed the spectral pattern of the 35% modified PEG-oGH, as compared with native oGH, did not change except for a slight decreased fluorescent intensity; the spectral pattern of the 86% modified one changed a great deal. Rat hepatocytes binding assay in vivo showed both modified oGH decrease considerably their original binding capacity to the hepatocytes. |
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| Keywords: | ovine growth hormone modification with polyethylene glycol anti- genicity and bioactivity fluorescant spectrum binding assay in vivo |
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