MMP-9和PEDF在胃癌侵袭转移中的作用

目的探讨基质金属蛋白酶9（matrix metalloproteinase 9，MMP-9）和色素上皮源性因子（pigment epithelium-derived factor，PEDF）基因表达在胃癌侵袭转移中的作用，为防治胃癌转移提供理论依据。方法应用免疫组织化学法检测82例人体胃癌组织标本中胃癌细胞内MMP-9和PEDF的表达；统计学分析两种分子标记物与胃癌侵袭转移关系。结果胃癌组织中的MMP-9和PEDF表达率分别约为70％和43％，MMP-9高表达和PEDF低表达与浸润深度、淋巴管转移和远处转移呈正相关（P〈0、05）；MMP-9与PEDF表达呈负相关（P〈0、01）。结论MMP-9在胃癌侵袭转移中起促进作用，PEDF可抑制胃癌的进展，PEDF低表达可能通过上调MMP-9的表达从而促进胃癌的得袭转移．

Expressions of MMP-9 and PEDF and their correlation with tumor invasion and lymph node metastasis in gastric carcinoma

Objective To investigate the expressions of Matrix Metalloproteinase 9(MMP-9) and Pigment epithelium-derived factor(PEDF) and their correlation with tumor invasion and metastasis in human gastric carcinoma.Methods Immunohistochemistry method was used to detect the expressions of MMP-9 and PEDF in 82 cases of gastric carcinoma tissue samples.The relationship between the molecular markers and tumor invasion and metastasis were analyzed.Results The expression rates of MMP-9 and PEDF were 70% and 43% respectively in gastric carcinoma patients.MMP-9 expression was related to depth of invasion and lymph node metastasis as well as distant metastasis.Low PEDF protein expression was significantly associated with tumor-increased penetration through the gastric wall,lymph node and distant metastasis.There was negative correlation between the expression of MMP-9 and PEDF.Conclusion MMP-9 can accelerate the development and progression of gastric carcinoma,while PEDF play a negative role in tumor progression.The low expression of PEDF may promote the tumor invasion by upregulating the expression of MMP-9.