疑似生殖器疱疹患者单纯疱疹病毒抗原及抗体检测与分析

目的 了解性病门诊疑似生殖器疱疹患者单纯疱疹病毒抗原及抗体检测情况.方法 对2008年4月至2012年3月到我院性病门诊就诊的1101名疑似GH患者进行HSV-2抗原检测,并对其中336名愿意同时采集血标本进行HSV-2血清抗体检测患者进行血清HSV-2IgM、HSV-2IgG抗体检测,对所检测结果及临床资料进行统计分析.结果 1101例疑似GH患者共检出551例HSV-2 DNA阳性,总阳性率50.05％,其中男性患者HSV-2 DNA阳性率为52.33％,女性患者HSV-2 DNA阳性率为40.99％,男女患者阳性率比较差异有显著性(P＜0.01);不同类型的标本HSV-2 DNA检出率有明显的差异,其中疱液拭子的检出率最高,为57.05％,其次是男性的阴茎拭子及包皮拭子,两者检出率分别为56.25％、46.62％;从年龄段来看,以20 ～ 29岁年龄段患者HSV-2抗原检出率最低,除该年龄段,其它年龄段阳性率均超过50％,≥50岁受检测患者,HSV-2抗原检出率高达56.21％.336位同时接受HSV-2 DNA及血清HSV-2IgM、HSV-2 IgG抗体检测患者中,共检出血清HSV-2 IgM抗体阳性33例,阳性率9.82％.血清HSV-2 IgG抗体阳性149例,阳性率44.35％,其中155名HSV-2 DNA阳性患者有104例至少一种抗体阳性,181名HSV-2 DNA阴性患者有61例至少一种抗体阳性.结论 对于疑似生殖器疱疹患者,同时进行HSV-2抗原及血清HSV-2 IgM、HSV-2 IgG抗体三者联合检测更有助于临床GH诊断;老年生殖器疱疹的防治应引起足够的重视.

Detection and analysis of herpes simplex virus antigens and antibodies in patients with suspected genital herpes

Objectives To explore the detection of antigens and antibodies of herpes simplex virus in patients with suspected genital herpes （ GH ）. Methods HSV-2 antigens were detected in 1101 patients with suspected GH who visited our sexually transmitted disease clinic from April 2008 to March 2012; HSV-2 IgM and HSV-2 IgG antibodies were also detected in 336 of the patients by using serologi- cal tests. The test results and clinical data were analyzed statistically, Results 551 of the 1101 patients were HSV-2 DNA positive （ 52.33% male and 40.99% female 7, with a total positive rate of 50.05% the positive rate differed statistically between male and female （ P〈 0.01 ）. The detection rate of HSV-2 DNA differed significantly among different ways to obtain specimens, in which swabs for blister fluid was the highest （ 57.05% ）, the highest of all, followed by penile swab test （ 56.25% ） and foreskin swab test （ 46.62% ）. The detection rate of HSV-2 antigens was lowest in patients aged 20 to 29; the positive rates were greater than 50% in other age groups, and the rate was up to 56.21% in those older than 50. Of 336 patients receiving both HSV-2 DNA test and HSV-2 IgM and HSV-2 IgG serum antibodies tests, 33 were HSV-2 IgM antibodies positive, with a positive rate of 9.82% 149 were HSV-21gG antibodies positive, with a rate of 44.35%. At least one antibody test was positive in 104 of the 155 HSV-2 DNA positive patients and at least one antibody test was positive in 61 of the 181 HSV-2 DNA negative patients. Conclusions For patients with suspected genital herpes, detection of HSV-2 antigen tests combined with HSV-2 IgM and HSV-2 IgG serum antibody tests is more helpful in the clinical diagnosis of genital herpes. We should pay more attention to the prevention and control of senile genital herpes.