白细胞介素-6基因启动-子572 C/G多态性与心绞痛的相关性研究

目的探讨白细胞介素-6(IL-6)基因启动子-572 C/G多态性各等位基因及基因型在心绞痛患者中的分布频率,并分析其基因型及血清水平与心绞痛的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测121例心绞痛患者及140例对照者IL-6基因启动子-572 C/G多态性,同时采用酶联免疫吸附试验(ELISA)检测心绞痛和对照者血清IL-6水平。结果心绞痛组IL-6血清水平非常显著高于对照组(P<0.01),IL-6基因启动子-572 C/G基因型频率和等位基因频率在心绞痛组和对照组间比较差异有显著性(P<0.05),等位基因频率的相对风险分析发现,G等位基因携带者患心绞痛的风险是C等位基因的1.665倍(OR=1.665,95%CI:1.089~2.544),携带G等位基因的心绞痛患者IL-6血清水平显著高于不携带者(P<0.05);结论IL-6基因启动子-572 C/G多态性与心绞痛的发病具有相关性,G等位基因可能是心绞痛发病的遗传易感基因,携带G等位基因的个体可能通过促进IL-6的高度表达进而增加心绞痛的发病风险。

Association of the IL-6 gene promoter -572C/G Polymorphism and angina pectoris

Objective To study the allele frequencies and genotype distribution of interleukin(IL-6)gene promoter-572C/G polymorphism in angina pectoris,And analyse the relationship between the serum levels and genotype of IL-6 with angina pectoris in Chinese populations.Methods The IL-6 gene promoter-572C/G polymorphism were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis among 121 patients with angina pectoris and 140 healthy controls,and the serum level of IL-6 was detected by enzyme-linked immunosorbent assay(ELISA).Results Angina pectoris group showed significantly higher serum levels of IL-6 than control group(P<0.01),There was significant difference in frequencies of allele and genotype in-572C/G polymorphism between angina pectoris and control groups respectively(P<0.05).The relative risk suffered from angina pectoris of C allele was 1.665 times of the G allele(OR=1.665,95%CI:1.089～2.544),the serum IL-6 level was significantly higher among carriers of G allele as compared with non-carriers(P<0.05).Conclusion IL-6 gene promoter-572C/G polymorphism was associated with angina pectoris,and G allele may be a genetic risk factor of angina pectoris.In which the IL-6 G allele carriers may be increase risk by enhancing the IL-6 expression in the pathogenesis of angina pectoris.