薄层扫描法测定香科属植物中4种新克罗烷型二萜的含量

目的：用薄层扫描法测定香科属植物中4种新克罗烷型二萜：teucvidin (Ⅰ), 19-acetyl-teuspinin (Ⅱ), teuscorodonin (Ⅲ), teupernin A (Ⅳ)。方法：样品经丙酮超声提取后，用XAD-2型大孔树脂去除色素；在高效硅胶薄层板上点样后展开，展开剂为乙酸乙酯—正己烷(6∶5)。所得斑点用Ehrlich试剂显色，扫描测定。结果：各新克罗烷型二萜的线性范围在0.63～2.18 μg，回收率为92.4%～97.8%，应用本法测定了香科属植物铁轴草、庐山香科丙酮浸膏中新克罗烷型二萜的含量。结论：本法简便易行，分析速度快，重现性好，可用于香科属植物中新克罗烷型二萜的测定。为开发资源，评价药物质量，提供了科学依据。

DETERMINATION OF FOUR NEO-CLERODANE DITERPENOIDS OF TEUCRIUM SPECIES BY HPTLC SCANNING

AIM: To establish a TLC scanning method for the separation and determination of the four neo-clerodane diterpenoids: teucvidin (Ⅰ), 19-acetyl-teuspinin (Ⅱ), teuscorodonin (Ⅲ) and teupernin A (Ⅳ) in T.quadrifarium and the acetone extract of T.pernyi from Teucrium species. METHODS: The sample was extracted with supersonic waves with acetone. Then XAD-2 macroporous resin was used to remove the pigment. EtOAc — n-hexane (6∶5) was used as developing solvent on the HPTLC silica gel plate. After that, the spots were identified by Ehrlich reagent and then determined with a Shimadzu CS-930 TLC scanner. RESULTS: The calibration curves were linear in the range of 0.63～2.18 μg for the four neo-clerodane diterpenoids and the recoveries were 92.4%～97.8%. The method had been applied to the analysis of two plants. CONCLUSION: The method is simple, feasible, rapid and reproducible. It offered a scientific basis for seeking and utilizing botanic resources, and evaluating the quality of herb medicines.