市售鹿鞭的DNA指纹鉴定研究

目的：探讨鹿鞭及牛鞭线粒体细胞色素b基因（Cytb）部分序列片段特征，建立中药材鹿鞭敏感、特异的DNA分子标记学鉴定方法。方法对市售鹿鞭进行样本处理，模板制备，采用鹿鞭特异性引物进行PCR反应，对市售鹿鞭样本采取DNA指纹鉴定。结果对市售鹿鞭中药材进行mtDNA鉴定，有三个样本为正品。结论此方法对市售鹿鞭鉴定，可准确辨别正品与伪品。重现性、稳定性好，简便准确而可行，说明鹿鞭mtDNA具有特异性指纹特征，所得鹿鞭DNA指纹特征图谱可用于市售鹿鞭的鉴定。

The identification of mitochondrial DNA finger print on the testis et penis cervi on sale

Objective To explore the identification and characterization of cytochrome b gene from the Testis et Penis Cervi and to develop a method of DNA molecular marker for identification of traditional Chinese medicine,the Testis et Penis Cervi. Methods Based on the analysis of DNA sequence of mitochondrial cytochrome b gene from fresh animals of the Testis et Penis Cervi and the bullwhip. A pair of allele-specific primers was designed for distinguishing the genuine drugs from their adulterants with primer design software Primer Premier 5.0. The fresh Testis et Penis Cervi and the bullwhip were extracted and polymerase chain reaction（PCR） amplification was carried out. The DNA bands of Testis et Penis Cervi were recovered,purified and sequenced.Commercially available six samples were identificated. Results The results of diagnostic PCR for original animals showed that a 306bp fragment was only amplified from DNA templates of the Testis et Penis Cervi. Only 3 samples were genuine drug. Conclusion DNA is specific finger print feature which can be used for identification and analysis between the Testis et Penis Cervi and the bullwhip. The method is simple and the result is reliable. It can be used for the crude drugs in the current market.