Multipotent and Committed CD34+ Cells in Bone Marrow Transplantation |
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Authors: | Hideo Ema Toshio Suda Hiromitsu Nakauchi Yukio Nakamura Atsushi Iwama Shigehiko Imagawa Miyuki Akutsu Yasuhiko Kano Shunichi Kato Miharu Yabe Minoru Yoshida Shinobu Sakamoto Youichi Amemiya Yasusada Miura |
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Institution: | Division of Hematology, Department of Medicine, Jichi Medical School, Minamikawachi-machi, Tochigi-ken 329-04;Laboratory of Molecular Regulation of Aging, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Kouyadai, Tsukuba-shi, Ibaraki-ken 305;Tochigi Cancer Center, Younan, Utsunomiya-shi, Tochigi-ken 320;Department of Pediatrics, School of Medicine, Tokai University, Bouseidai, Isehara-shi, Kanagawa-ken 259-11 |
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Abstract: | In order to study the role of CD34+ cells in hematological recovery following bone marrow transplantation (BMT), bone marrow cells stained with HPCA-1 (CD34) and MY-9 (CD33) monoclonal antibodies were analyzed by using a fluorescence-activated cell sorter on or about days 14 and 28, as well as at later times, following BMT in 6 recipients. Single cell cultures of CD34+ cells were also performed to evaluate their in vitro hematopoietic function. CD34+ cells were detectable in bone marrow cells on day 14. More than 80% of CD34+ cells co-expressed the CD33 antigen, and macrophage (Mac) colony-forming cells predominated among total colony-forming cells of CD34+ cells. In normal bone marrow cells, CD34+, CD33+ cells amounted to about 40% of CD34+ cells, and the incidences of erythroid bursts, granulocyte/macrophage (GM) colonies, and Mac colonies were similar to each other. After more than 10 weeks, CD34+, CD33? cells gradually recovered, as erythroid burst colony-forming cells increased following GM colony-forming cells. This phenomenon was well-correlated with the time course of peripheral blood cell recovery. CD34+, CD33+ cells as committed progenitors and CD34+, CD33? cells as multipotent stem cells have distinctive biological behaviors in BMT. |
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Keywords: | Bone marrow transplantation CD34+ cells Hematopoietic stem cells |
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