不同病程糖尿病大鼠肾脏一氧化氮变化与氧自由基的关系

为探讨糖尿病(DM) 大鼠肾脏一氧化氮(NO) 变化与氧自由基的关系,测定链脲佐菌素(STZ) 诱导的DM 大鼠及对照组(NC) 于病程2 ,8 ,16 周时肾组织NO 含量和一氧化氮合酶(NOS) 活性,脂质过氧化物(LPO) 水平及超氧化物歧化酶(SOD) 、过氧化物酶(POD) 和过氧化氢酶(CAT) 活性变化。结果表明:DM 大鼠肾脏NO 水平及NOS活性随病程的延长由升高至下降;各病程DM 大鼠肾脏LPO 水平均高于同批NC 组,8与16 周时,肾组织SOD,POD,CAT 活性低于同批NC 组。提示STZ 诱导的DM 大鼠肾脏NO 水平的动态变化可能与高血糖所致的肾脏抗氧化机能改变有关

Relationship between nitric oxide and oxygen free radicals in different duration of diabetes in rat kidney

To study the relationship between nitric oxide(NO) and oxygen free radicals in rat kidney of diabetes mellitus(DM), the authors examined the changes of renal tissue NO level and nitric oxide synthase(NOS) activity, lipid peroxidation(LPO) level and superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) activities in streptozotocin(STZ) induced diabetic rats and normal controls(NC) at the 2nd, 8th and 16th week. The results showed that renal tissue NO level and NOS activity were increased at the 2nd week, but decreased at the 8th and 16th week; LPO levels were significantly elevated in all DM groups than those in NC groups. SOD, POD and CAT activities dcreased significantly in the 8th and 16th week DM groups compared with those in NC groups. The results suggest that NO level in kidney of STZ induced diabetic rats dynamically change with the disease course of dvelopment. This change may be related with the change of antioxidation in kidney which high glucose induces.