| 普通TaqMan探针及raqMan MGB探针real-time PCR检测无形体msp-2基因方法的建立与比较 |
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| 引用本文： | 王誓闻,张丽娟,王园园,禹惠兰,梁长威,陈强,于强. 普通TaqMan探针及raqMan MGB探针real-time PCR检测无形体msp-2基因方法的建立与比较[J]. 疾病监测, 2011, 26(1): 12-14. DOI: 10.3784/j.issn.1003-9961.2011.01.005 |
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| 作者姓名： | 王誓闻 张丽娟 王园园 禹惠兰 梁长威 陈强 于强 |
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| 作者单位： | 1.中国疾病预防控制中心传染病预防控制所,北京 102206 |
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| 基金项目： | 国家基础研究项目-973计划( No.2010CB530200,2010CB530206); 传染病防治科技重大专项( No.2009ZX10004 -203,2008ZX10004 -008)~~ |
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| 摘 要： | 目的 建立敏感、特异、定量real-time PCR方法,用于无形体病早期诊断及立克次体病鉴别诊断.方法 通过GenBank Blast比较分析,选择世界各地39株无形体,以msp-2特异基因保守区设计普通TaqMan探针及TaqMan MGB探针并比较分析.结果 普通TaqMan探针及TaqMan MGB探针2种方法...
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| 关 键 词： | 无形体 荧光定量PCR 普通TaqMan探针 TaqMan MGB探针 |
| 收稿时间： | 2010-09-15 |
Establishment and comparison of real-time PCR assays to detect Anaplasma msp-2 gene with general TaqMan probs and TaqMan-MGB probe |
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| WANG Shi-wen,ZHANG Li-juan,WANG Yuan-yuan,YU Hui-lan,LIANG Chang-wei,CHEN Qiang,YU Qiang. Institute for Communicable Disease Prevention , Control,Chinese Center for Disease Control , Prevention,Beijing ,China. Establishment and comparison of real-time PCR assays to detect Anaplasma msp-2 gene with general TaqMan probs and TaqMan-MGB probe[J]. Disease Surveillance, 2011, 26(1): 12-14. DOI: 10.3784/j.issn.1003-9961.2011.01.005 |
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| Authors: | WANG Shi-wen ZHANG Li-juan WANG Yuan-yuan YU Hui-lan LIANG Chang-wei CHEN Qiang YU Qiang. Institute for Communicable Disease Prevention Control Chinese Center for Disease Control Prevention Beijing China |
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| Affiliation: | 1.Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective In order to develop a highly specific and sensitive real-time PCR assay to detect and indentify Anaplasma,the agent of Anaplasmoses. Methods A specific msp-2 outer membrane protein gene of A.phagocytophilum was selected for designing general TaqMan probe and TaqMan MGB probe based on the conserved sequences of msp-2 genes of 39 isolates all over the world. The evaluation of assay included the specificity,lowest detection load (LOD) and stability. Results Similar specificity and reproducibility for... |
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| Keywords: | Anaplasma real-time PCR general TaqMan probe TaqMan-MGB probe |
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