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| 气相色谱法测定燕窝中醛糖的含量 | |
| 引用本文： | 王慧,王玉,倪坤仪. 气相色谱法测定燕窝中醛糖的含量[J]. 中国药学杂志, 2006, 41(14): 1108-1110 |
| 作者姓名： | 王慧 王玉 倪坤仪 |
| 作者单位： | 1. 中国药品生物制品检定所,北京,100050 2. 江苏省药品检验所,南京,210008 3. 中国药科大学,南京,210009 |
| 摘 要： | 目的建立燕窝中4种醛糖的分离分析方法。方法燕窝中的4种醛糖首先在2 mol·L-1的盐酸甲醇中水解20 h,用氢氧化钾-甲醇中和后再在吡啶乙酸酐溶液中乙酰化。采用毛细管气相色谱法,HP-5柱,程序升温法进行测定。结果甘露糖、半乳糖、N-乙酰半乳糖和N-1内线性良好;平均回收率分别为95.7%,96.1%,95.1%和97.0%。结论本方法具有分辨率好、灵敏度高等特点,同时也为真假燕窝的鉴别提供了一种行之有效的方法。 |
| 关 键 词： | 燕窝 半乳糖 甘露糖 N-乙酰葡萄糖 N-乙酰半乳糖 |
| 文章编号： | 1001-2494（2006）14-1108-03 |
| 收稿时间： | 2005-07-20 |
| 修稿时间： | 2005-07-20 |
Determination of Aldoses in Edible Bird''''s Nest by Gas Chromatography |
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| WANG Hui,WANG Yu,NI Kun-yi. Determination of Aldoses in Edible Bird''''s Nest by Gas Chromatography[J]. Chinese Pharmaceutical Journal, 2006, 41(14): 1108-1110 | |
| Authors: | WANG Hui WANG Yu NI Kun-yi |
| Affiliation: | 1. National Institute for the Control of Phamaceutical and Biological Products, Beijing 100050, China;2. Jiangsu Institute for Drug Control,Nanjin 210008, China; 3.China Pharmaceutical University, Nanjing 210009, China |
| Abstract: | OBJECTIVE To determine the contents of four aldoses in edible bird's nest(EBN).METHODS Firstly,the sample was hydrolyzed by 2 mol·L-1 HCl-methanol and neutralized by 2 mol·L-1 potassium hydroxide,then derivated by acetic anhydride in pyridine.Four aldoses were seperated completely using HP-5 capillary column and programablly increased temperature.RESULTS The linear range of D-Mannose,D-Galactose,N-Acetyl-D-Galactosamine and N-Acetyl-D-Glucosamine were 0.032 2～0.161,0.316～1.58,0.104～0.520 and 0.212～1.06 mg·L-1,respectively.The average recovery was 95.7%,96.1%,95.1% and 97.0%,respectively.CONCLUSION A validated GC method is established to determine four aldoses.The method is specific and sensitive. |
| Keywords: | edible bird's nest D-galactose D-mannose N-acetyl-D-galactosamine N-acetyl-D-glucosamine |
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