RP-HPLC测定人尿液中甲磺酸帕珠沙星的浓度 |
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引用本文: | 徐文泉,赵勤,何思润,董奇星,秦群.RP-HPLC测定人尿液中甲磺酸帕珠沙星的浓度[J].中国当代医药,2014,21(8):9-11. |
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作者姓名: | 徐文泉 赵勤 何思润 董奇星 秦群 |
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作者单位: | 徐文泉 (北京航空航天大学体育部,北京,100191); 赵勤 (中南大学湘雅医院,长沙,410008); 何思润 (中南大学湘雅医院,长沙,410008); 董奇星 (中南大学湘雅医院,长沙,410008); 秦群 (中南大学湘雅医院,长沙,410008); |
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基金项目: | 中南大学湘雅医院校级横向课题 |
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摘 要: | 目的 建立测定甲磺酸帕珠沙星的尿药浓度的方法,计算累积尿药排泄率.方法 采用反相高效液相色谱(RP-HPLC)法,色谱柱为C18柱(4.6 mm×150 mm,5μm),柱温35℃;流动相:乙腈-甲醇-水(6∶12∶82,含0.01%磷酸和0.01%三乙胺);流速:1.10 ml/min;检测波长:245 nm.结果 甲磺酸帕珠沙星最低定量浓度为13.6 μg/ml;尿药标准曲线线性范围为13.6~2720 μg/ml (r=0.9997),方法回收率>90%;日内、日间精密度(RSD)<10.0%.30名健康志愿者进行单剂量静脉注射低、中、高三种剂量的注射用甲磺酸帕珠沙星后,帕珠沙星在24 h内尿液中的累积尿药排泄率分别为(92.63±5.04)%、(91.21±2.09)%、(90.22±3.53)%.结论 该方法快速、简便、准确度高,专属性好,无杂质干扰,适用于帕珠沙星体内药物浓度的测定及药代动力学研究.
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关 键 词: | 甲磺酸帕珠沙星 反相高效液相色谱法 尿药浓度 |
Determination of pazufloxacin mesilate in human urine by RP-HPLC |
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Authors: | XU Wen-quan ZHAO Qin HE Si-run DONG Qi-xing QIN Qun |
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Institution: | XU Wen-quan ZHAO Qin HE Si-run DONG Qi-xing QIN Qun |
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Abstract: | Objective To establish a method to determine the urine concentration of pazufloxacin mesilate and calcu- late cumulative urinary excretion rate.Methods Reversed phase high performance liquid chromatography (RP-HPLC) was established,the column was Cts (4.6 mm150 mm,5 μm),column temperature was 35C,with acetonitrile-methanol-water (0.01% H3PO4 and 0.01% triethyamin) of 6:12:82 as the mobile phase.The flow rate was 1.10 ml/min.The detection wavelength was 245 nm.Results The calibration curve was linear in the range of 13.6 to 2720 pg/ml (r=0.9997) for pazu- floxacin.The methodology recoveries was higher than 90% for the anahytes.The RSD value of within-day and between- day was less than 10.0%.After being given an iv administration of single dose including low,medium and high of pazu- floxacin mesilate,the accumulative urine exeretion rate of 30 volunteers during 24 h was (92.63+_5.04)%,(91.21_+2.09)%, (90.22+3.53)% respectively.Conclusion The method is accurate,sensitive,specific and reliable for pazufloxacin,and the impurities do not interrupt.It may be applied to study drug concentration and pharmacokinetic. |
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Keywords: | Pazufloxacin mesilate Reversed phase high performance liquid chromatography Urine concentration |
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