| HPLC法测定柴葛解肌汤中葛根素的含量 |
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| 引用本文： | 毕何锋,张婕,蒋东旭,谢友良,赖小平.HPLC法测定柴葛解肌汤中葛根素的含量[J].中国药师,2014(1):161-162. |
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| 作者姓名： | 毕何锋 张婕 蒋东旭 谢友良 赖小平 |
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| 作者单位： | 广州中医药大学 广州 510006；;广州中医药大学 广州 510006；;广州中医药大学 广州 510006；东莞广州中医药大学中医药数理工程研究院;广州中医药大学 广州 510006；东莞广州中医药大学中医药数理工程研究院;广州中医药大学 广州 510006；东莞广州中医药大学中医药数理工程研究院 |
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| 基金项目： | 广东省高新区发展引导项目（编号2011B010600045） |
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| 摘 要： | 目的：建立反相高效液相色谱法测定柴葛解肌汤中葛根素的含量.方法：采用Hypersil BDS C18色谱柱,以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速为1.0 ml·min^-1,检测波长250 nm,柱温30℃.结果：葛根素进样量在0.032 3～0.323 2 μg范围内呈良好的线性关系（r=0.999 8,n=6）;平均加样回收率为101.4%,RSD为2.9%（n=6）.结论：该法具有简便易行、检测灵敏、重复性好等优点,可用于柴葛解肌汤中葛根素的含量测定.
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| 关 键 词： | 柴葛解肌汤 葛根素 高效液相色谱法 含量测定 |
| 收稿时间： | 6/5/2013 12:00:00 AM |
| 修稿时间： | 2013/10/10 0:00:00 |
Determination of Puerarin in Chaige Jieji Decoctions by HPLC |
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| Bi Hefeng,Zhang Jie,Jiang Dongxu,Xie Youliang and Lai Xiaoping.Determination of Puerarin in Chaige Jieji Decoctions by HPLC[J].China Pharmacist,2014(1):161-162. |
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| Authors: | Bi Hefeng Zhang Jie Jiang Dongxu Xie Youliang and Lai Xiaoping |
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| Institution: | Guangzhou University of TCM ,Guangzhou 510006,China;;Guangzhou University of TCM ,Guangzhou 510006,China;;Guangzhou University of TCM ,Guangzhou 510006,China;Donggan Mathematical Engineering Acaderuy of Chinese Nsedicine, Guangzhou University of TCM;Guangzhou University of TCM ,Guangzhou 510006,China;Donggan Mathematical Engineering Acaderuy of Chinese Nsedicine, Guangzhou University of TCM;Guangzhou University of TCM ,Guangzhou 510006,China;Donggan Mathematical Engineering Acaderuy of Chinese Nsedicine, Guangzhou University of TCM |
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| Abstract: | Objective： To establish a method for the determination of Puerarin in Chaige Jieji decoctions by HPLC. Methods： The separation was carried on a Hypersil BDS C18 column with acetonitrile-0.1% phosphoric acid as the mobile phase with gradient elution, and the column temperature was maintained at 30℃. The detecting wavelength was 250 nm, and the flow rate was 1.0 ml ·min^-1 Results： The linearity of the calibration curve was good within the range of 0.032 3-0.323 2μg for puerarin. The average reeoverywas 101.4% （RSD = 2.9% ,n = 6）. Conclusion： The method can sensitively detect puerarin with a simple pretreatment, and has a broad linear range with good repeatability, which can be used in the determination of puerarin in Chaige Jieji decoctions. |
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| Keywords: | Chaige Jieji decoctions Puerarin HPLC Content determination |
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