丝切蛋白在非小细胞肺癌中的表达及其临床意义

[日的]探讨丝切蛋白（cofilin）住非小细胞肺癌（NSCLC）组织和不同转移潜能肺癌细胞系中的表达及其临床意义。[方法]应用Westernblot法检测肺癌细胞系中cofilin蛋白的表达水平：并采用免疫组织化学SP法检测100例NSCLC组织及30例癌旁正常组织中cofilin的表达。[结果]高转移性肺癌细胞系中cofilin蛋白的表达明显高于低转移性肺癌细胞系。100例NSC!．C组织中，cofilin的阳性表达率为65％。cofilin蛋白的表达与NSCLC患者的性别、年龄、肿瘤大小、组织类型、临床分期以及病理分级均无关（P均〉O．05），仅与有无淋巴结转移有关（火0．01）。[结论]cofilin蛋白在NSCI．C的生长和转移过程中发挥着重要的调控作用．可作为判断NSCI。C转移的重要生物学指标。

Expression of Cofilin in Non-small Cell Lung Cancer and Its Clinical Significance

[Purposel To investigate the expression of cofilin in human non- small cell hmg cancer tis- sues （NSCLC） and lung cancer cell lines,and its clinical significance with eancerous metastasis. [Metbods] The expression of cofilin protein in lung cancer cell line was detected by Western blot. Cofilin was detected by immuno- histochemical staining SP technique in tumor tissues from 100 lung cancer cases and in carcinoma adjacent tissues from 30 patients. [Results] The expression of cofilin protein up-regulated in highly metastatic human lung caneer. The positive rate of cofilin expression in 100 lung cancer eases was 65%. The up-regulation of cofilin expression significantly correlated with lymph node metastasis,but did not correlate with age,sex,tumor size,histological type,TNM stage and pathological grading. [Conclusion] Cofilin probably plays an important regulating role in the growth anti metastasis tff lung cancer. Detection of cofilin expression can help in evaluating the metastasis of non-small cell lung cancer.