| HPLC法测定大鼠肝微粒体中6-羟基氯唑沙宗的含量 |
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| 引用本文： | 唐景玲,周小燕,徐娜,孙立欣,纪宏宇,刘红梅,吴琳华. HPLC法测定大鼠肝微粒体中6-羟基氯唑沙宗的含量[J]. 中国药师, 2014, 0(1): 53-55 |
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| 作者姓名： | 唐景玲 周小燕 徐娜 孙立欣 纪宏宇 刘红梅 吴琳华 |
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| 作者单位： | 哈尔滨医科大学药学院 哈尔滨 150086；;哈尔滨医科大学药学院 哈尔滨 150086；;哈尔滨医科大学药学院 哈尔滨 150086；;哈尔滨医科大学药学院 哈尔滨 150086；;哈尔滨医科大学附属第二医院药学部；;哈尔滨医科大学附属第二医院药学部；;哈尔滨医科大学附属第二医院药学部；黑龙江省高校重点实验室 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目（编号: 11551274） |
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| 摘 要： | 目的：建立高效液相色谱法测定大鼠肝微粒体中6-羟基氯唑沙宗的含量.方法：采用Diamonsil C18柱（200 mm×215;4.6 mm,5 μm）,以甲醇-水-磷酸（45：55：0.1）为流动相,流速1.0 ml·min^-1,检测波长为296 nm.结果：6-羟基氯唑沙宗浓度在0.5 - 25 μmol·L^-1 范围内呈现良好的线性关系（r=0.999 1）,方法回收率为92.57% - 98.72%,提取回收率为76.38%-82.25%,日内、日间精密度RSD〈9%.结论：该方法可靠、准确、重复性好,可用于大鼠肝微粒体中6-羟基氯唑沙宗的含量测定及体外代谢研究.
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| 关 键 词： | 高效液相色谱法 6-羟基氯唑沙宗 肝微粒体 药物代谢 |
| 收稿时间： | 2013-07-03 |
| 修稿时间： | 2013-10-10 |
Determination of 6-Hydroxy Chlorzoxazone in Rat Liver Microsomes by HPLC |
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| Tang Jingling,Zhou Xiaoyan,Xu N,Sun Lixin,Ji Hongyu,Liu Hongmei and Wu Linhua. Determination of 6-Hydroxy Chlorzoxazone in Rat Liver Microsomes by HPLC[J]. China Pharmacist, 2014, 0(1): 53-55 |
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| Authors: | Tang Jingling Zhou Xiaoyan Xu N Sun Lixin Ji Hongyu Liu Hongmei Wu Linhua |
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| Affiliation: | College of Pharmacy, Harbin Medical University, Harbin 150086, China;;College of Pharmacy, Harbin Medical University, Harbin 150086, China;;College of Pharmacy, Harbin Medical University, Harbin 150086, China;;College of Pharmacy, Harbin Medical University, Harbin 150086, China;;College of Pharmacy, Harbin Medical University, Harbin 150086, China; 2.Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University; 7. Key Laboratory of Drug Research, College of Heilongjiang Province;Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University;;Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University; Key Laboratory of Drug Research, College of Heilongjiang Province |
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| Abstract: | Objective： To establish an HPLC method for the determination of 6-hydroxy chlorzoxazone in rat liver microsomes. Methods： The samples were separated on a RP-Cjs column （Diamonsil, 200 mm × 4.6 mm, 5 μm） with methanol-water-phosphoric acid （45： 55：0.1 ） as the mobile phase at a flow rate of 1.0 ml ·min^-1 and the detection wavelength was at 296 nm. Results： The calibration curve was linear over the range of 0.5-25 μmol·L^-1 for 6-hydroxy chlorzoxazone （ r = 0.999 1 ）. The relative recovery of the samples ranged from 92.57% -98.72%. The absolute recovery of the samples ranged from 76.38% -82.25%. The intra and inter- day precision RSD were below 9%. Conclusion： The method is reliable, precise and reproducible in the content determination of 6-hy- droxy chlorzoxazone in rat liver microsomes and metabolism in vitro. |
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| Keywords: | HPLC 6-Hydroxy chlorzoxazone Liver microsomes Drug metabolism |
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