神经生长因子对2,5-己二酮诱导的PC12细胞凋亡的影响

目的建立大鼠肾上腺嗜铬细胞癌PC12细胞2,5-己二酮(2,5-HD)中毒模型,观察研究神经生长因子(NGF)对2,5-HD中毒的保护作用。方法将细胞随机分为对照组(于正常培养基生长)、HD组(不同浓度2,5-HD)、NGF HD组(不同浓度NGF 20mmol/L,2,5-HD),采用噻唑蓝(MTT)法检测细胞存活率。结果随着2,5-HD处理浓度升高和时间延长,细胞损伤加重,存活率较对照组逐渐降低(P<0.05,P<0.01);以NGF预处理的细胞显示出明显的抗损伤性,细胞存活率较HD组逐渐提高(P<0.05,P<0.01)。结论2,5-HD呈剂量性抑制PC12细胞存活和增殖,诱导细胞损伤和凋亡,NGF对2,5-HD中毒性PC12细胞显示出明显保护作用。

The Effect of Nerve Growth Factor on P12 Cells Withdrawing Inducted by 2,5-Acetyl Butyryl

Objective: To set up a model of 2,5-HD intoxication of PC12 Cells of Rats' pheochromocytoma of adrenal;to observe and research the protective effect of nerve growth factor on 2,5-HD intoxication.Methods: Cells are divided by random into control group(maintained in normal medium) and HD group(processed by 2,5-HD) and NGF THD group processed by different concentration of NGF 20mmol/L,2,5-HD).MTT is adapted to detect the cells survivor rate.Results: With the increasing of 2,5-HD concentration and the time extension,the cell injuries become worse and the survivor rate decreases gradually(p<0.05);the cells pre-processed by NGF show significant anti-injuring property and the survivor rate rises gradually(p<0.05),and NGF shows good protective effect on dose-dependent manner to protect PC 12(P<0.001).Conclusions: 2,5-HD has the property of dose-dependent manner to inhabit the survival and hyperplasia of PC 12,induct cells injuries and withdrawing;NGF has obvious protective effect on 2,5-HD intoxication of PC 12 cells.