普伐他汀促进对慢性脑缺血β淀粉样蛋白的清除

目的观察降血脂药普伐他汀对慢性脑缺血大鼠的β淀粉样蛋白(Aβ)水平的影响。方法39只雄性SD大鼠分为正常饮食组(6只),高脂饮食组随机分为空白对照组(7只)、普伐他汀服用组(7只)、单纯脑缺血组(9只)及普伐他汀加脑缺血组(10只),采用结扎双侧颈总动脉建立CVI模型,于术后1个月行为学试验后,测血脂及Aβ水平。取脑组织,行病理学分析及ELISA检测Aβ水平。结果未治疗组(空白对照、单纯脑缺血组)血脂水平升高,但无显著差异(p>0.05),治疗组为正常水平。与正常饮食组相比,其余各组均无明显运动功能障碍,第4周平台定位时间、搜索策略无显著性差异(p>0.05)。脑病理学呈慢性缺血性改变结构欠清晰,神经元固缩或均匀着色,但明显的细胞坏死改变较少见;白质疏松明显。ELISA显示单纯缺血组Aβ较对照组明显升高,而治疗组Aβ下降至略高于对照组水平。结论普伐他汀能够降低脑内Aβ水平,这种降脂外的调节Aβ代谢作用提示血脂代谢异常参与了AD发病。

PRAVASTATIN ACCELERATING THE CLEARANCE OF β AMYLOID PROTEIN DURING CHRONIC CEREBROVASCULAR ISCHEMIA

Objective To investigate the influences of pravastatin on the chronic cerebral ischemia rats. Methods Based on permanent bilateral occlusion of both common carotid arteries (2-VO) model,thirty-nine S-D rats were divided to normal diet group,and high-fat diet group which were sub-divided into control group,pravastatin,2-VO,2-VO+pravastatin groups. Animals were tested by Morris Water Maze,sacrificed at 30 days and the brain were processed for neuropathology or ELISA analysis. Results There is no significant difference of serum lipids between groups,nor of memory function. Compared to the control groups,all 2-VO groups had typical chronic ischemic changes in brains. More importantly,Pravastatin can significantly decrease the Aβ level in brain tissue. Conclusions Pravastatin could be protective for dementia for its specific function on clearance of Aβ.