COX-2和VEGF在子宫腺肌病组织中的表达及其意义

目的:观测子宫腺肌病异位子宫内膜组织COX-2和VEGF表达及其临床意义。方法:34例经病理证实为子宫腺肌病标本为研究组,30例非子宫腺肌病子宫标本为对照组。采用免疫组化法检测研究组原位内膜和腺肌病病灶、对照组内膜和子宫平滑肌层中COX-2和VEGF的表达。采用RT-PCR检测COX-2mRNA和VEGFmRNA的表达。结果:COX-2和VEGF在研究组原位内膜和腺肌病病灶中的表达率均高于对照组内膜,研究组腺肌病病灶表达率均高于原位内膜(P<0.01)。增殖期和分泌期二者在腺肌病病灶表达率均无统计学差异(P>0.05)。分泌期二者在研究组和对照组中内膜的表达均高于增殖期(P<0.05)。COX-2和VEGF蛋白表达存在线性相关(r=0.787,0.562,P<0.01)。RT-PCR检测表明,COX-2mRNA在研究组腺肌病病灶存在高表达,对照组肌层和内膜不存在COX-2mRNA表达。比较对照组正常肌层,腺肌病病灶中VEGF165和VEGF121呈现高表达(P<0.01)。结论:COX-2和VEGF的高表达可能与子宫腺肌病的发生、发展有关。

The expression and clinical significance of COX-2 and VEGF in the women with adenomyosis

Objective:To investigate the expression and clinical significance of cycloxygenase(COX-2) and vascular endothelial growth factor(VEGF) in the women with adenomyosis.Methods:34 cases diagnosed as adenomyosis by pathological examination were research group,and 30 cases diagnosed as uterine myoma were control group.The immunohistochemistry staining was used to observe the expression of COX-2 and VEGF in endometrium of research group and control group.The expressions of COX-2 mRNA and VEGF mRNA were measured by RT-PCR.Results:The expression rates of COX-2 and VEGF protein in eutopic and ectopic endometrium of research group were both higher than those in eutopic endometrium of control group(P<0.01).The expression rates of COX-2 and VEGF protein in ectopic endometrium were higher than those in eutopic endometrium of research group(P<0.01).The expression rate in ectopic endometrium of research group had no significant difference(P>0.05) between the proliferative phase and the secretary phase.The expression of COX-2 and VEGF protein were linearly dependent(γ=0.787,γ=0.562,P<0.01).There was obvious COX-2 mRNA expression in ectopic endometrium in research group and there wasn't COX-2 mRNA expression in the normal muscle layer and endometrium in control group.The expression rates of VEGF165 and VEGF121 in the ectopic endometrium in research group were significantly higher than those in control group(P<0.01).Conclusion:The high expression of COX-2 and VEGF in the ectopic endometrium of the adenomyosis women may play an important role in the genesis and development of adenomyosis.