达英-35预防环磷酰胺对大鼠卵巢功能损伤的实验研究

目的:研究口服避孕药达英-35在雌性大鼠中减轻环磷酰胺(CTX)诱导的性腺毒性的效果。方法:随机将75只雌性Wistar大鼠分为5组:空白对照组、CTX组、达英-35组、联合治疗Ⅰ组和联合治疗Ⅱ组。用放射免疫法测定各组雌二醇(E2)和卵泡刺激素(FSH)水平,并于停药当天、第15天、30天,处死各组1/3的大鼠,比较卵巢重量、卵泡数量变化。结果:停药第30天,CTX组大鼠E2为(21.88±2.10)pg/ml,明显低于两个联合治疗组,FSH为(7.05±0.18)mIU/ml,明显高于两个联合治疗组,差异均有统计学意义(P0.05);CTX组卵巢重量和卵泡总数分别为(32.40±5.55)mg和308±32个,明显低于两个联合治疗组,差异有统计学意义(P0.05);联合治疗Ⅰ、Ⅱ组的E2、FSH、卵巢重量、卵泡数量分别比较,差异无统计学意义(P0.05)。结论:达英-35降低了CTX对大鼠卵巢功能的损伤,对卵巢功能的保护和恢复起到了一定的作用。

Experimental study of the prevention of Diane-35 cyclophosphamide-induced ovarian damage in rat model

Objective:To study the effect of oral contraceptives(OC) Diane-35 against cyclophosphamide(CTX)induced gonadotoxicity in the female rats.Methods:75 female Wistar rats were divided into five groups:blank control group,CTX group,Diane-35 group,combination groupⅠ,combination groupⅡ.E2,FSH were measured by RIA,and one-third of rats in each group were killed respectively on the stop day,15 days,30 days to compare the weight of ovary and the number of follicles.Results:On stop 30 days,E2 of CTX group was (21.88±2.10)pg/ml,lower than that of combination groups(P<0.05),FSH was(7.05±0.18)mIU/ml,higher than that of combination groups(P<0.05),ovary weight and total follicle number of CTX group were (32.40±5.55)mg and 308±32,obviously lower than those of combination groups(P<0.05).No significant differences were found between the two combination groups in E2,FSH,ovary weight and follicle number(P>0.05).Conclusion:Diane-35 may decrease CTX-induced ovarian damage in rat and plays a potential role in protecting and restoring ovarian function.