大鼠肺缺血再灌注损伤长期存活模型的建立

目的 建立一种简单、稳定的大鼠肺缺血再灌注损伤(LIRI)长期存活模型.方法 将84只健康SD大鼠随机分为2组:Ⅰ组为假手术组,Ⅱ组为肺缺血再灌注组(每组n=42只).两组分别于开胸后、缺血1 h后再灌注0、2、4h、1、3、7 d取肺组织行髓过氧化物酶(MPO)活性、肺湿干比(W/D ratio)检测和肺泡Ⅱ型细胞(ATⅡ)的电镜超微结构评价;取支气管肺泡灌洗液检测肺通透性指数(LPI).结果 手术成功率达100%.Ⅱ组与Ⅰ组比较,缺血再灌注后2、4 h、1 d的MPO、LPI、W/D比差异均有统计学意义(P<0.01),开胸后、再灌注后0 h、3、7 d两组比较差异无统计学意义(P>0.05).ATⅡ的超微结构显示,再灌注4 h后损伤最严重,再灌注1 d即出现明显的修复过程,再灌注7 d基本恢复正常结构水平.结论 本模型简单、可靠,LIRI后相关肺损伤指标和ATⅡ超微结构损伤变化特点吻合.

Establishment of a rat lung ischemia-reperfusion injury long-term survival model

Objective To establish a simple and stable rat lung ischemia-reperfusion (LIRI) long-term survival model. Methods Eighty-four healthy SD rats were randomly divided into two groups:Group Ⅰ,the sham-operated group (n=42, no hilar blocking);Group Ⅱ, lung ischemia reperfusion group (n=42,left hilar blocking). The lung tissues and blood samples were collected at baseline before hilar oc-clusion,and 0 h,2 h,4 h,1 day,3 days and 7 days after repeffusion. The indices were determined as fol-lows:the myeloperoxidase (MPO) activity, wet/dry ratio (W/D ratio), lung permeability index (LPI) and the ultrastructure of AT Ⅱ. Results All the SD rats survived during the observation period. Compared with group Ⅰ,the MPO activity, W/D ratio, and the LPI were significantly increased in group Ⅱ at the time points of 2 h,4 h, 1 day after reperfusion (all P<0.01). The ultrastructure of AT Ⅱ displayed that the most serious injury occurred at the fine point of 4 h after reperfusion. The repair process emerged at 1st day after reperfusion. At 7th day after reperfusion, the lung tissue structure almost recovered to normal. Conclu-sion It is a simple and reliable animal model. The related indices of lung injury were in accordance with the changes of ultrastruture of ATⅡ.