聪圣胶囊对去皮层血管大鼠行为学及前脑AchE、ChAT的影响

采用去大鼠脑皮层血管建立模型 ,AchE组化及ChAT免疫组化方法 ,研究聪圣胶囊对此模型皮质、海马以及大细胞基底核胆碱能神经元的影响。发现去脑皮层血管大鼠出现了明显的学习、记忆功能的损害。大鼠皮层损伤侧扣带皮质、海马CA1区AchE阳性纤维的分布明显稀疏 ,着色浅。损伤侧的大细胞基底核的AchE、ChAT阳性胆碱能神经元出现了明显萎缩。聪圣胶囊治疗组大鼠的学习、记忆功能明显改善 ,皮质、海马AchE阳性纤维分布较密集 ,着色加深。大细胞基底核AchE、ChAT阳性神经元的变性、萎缩明显减轻。说明聪圣胶囊对去皮层血管后造成的皮质、海马、大细胞基底核的损害具有明显的保护和治疗作用。通过上调胆碱能系统的功能改善学习、记忆功能。

Effects of Congsheng Capsules on the Behavior and Forebrain AchE and ChAT in the Rats with Cortical Devascularization

The rat model of vascular dementia was established by the cortical devascularization method. It was found in the rat model that learning and memorizing abilities were remarkably decreased, and the quantity of AchE positive fibers was significantly reduced in the cortex, the pyramidal cell layer of CA1 - CA4 subregions of the hippocampus, and in some other areas; and the quantity of Ach and ChAT positive neurons was obviously decreased in the MBN. In the rat model, atrophic cells, characterized by small sizes and less branches or even no any branches, were also found in the MBN. While in the Congsheng Capsules treated group, it was found that the learning and memorizing abilities were improved, the density of AchE positive fibers in the cortex and CA1 subregion of the hippocampus was obviously increased, and the AchE and ChAT positive neurons in the MBN were morphologically intact. The results of this study suggest that Congsheng Capsules have a protective effect against the injuries of neurons caused by the ischemia due to cortical devascularization, and an improving effect against the disturbance in learning and memorizing caused by cortical devascularization.