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| 鼠神经干细胞对胶质瘤C6细胞侵袭能力的影响 | |
| 引用本文： | 刘楠,杨俊荣,宋月佳,佟志国,宋菡姝,戚基萍. 鼠神经干细胞对胶质瘤C6细胞侵袭能力的影响[J]. 临床与实验病理学杂志, 2011, 27(11) |
| 作者姓名： | 刘楠 杨俊荣 宋月佳 佟志国 宋菡姝 戚基萍 |
| 作者单位： | 1. 哈尔滨医科大学附属第一医院病理科,哈尔滨,150001 2. 哈尔滨医科大学附属第一医院消化内科,哈尔滨,150001 3. 哈尔滨医科大学附属第四医院肿瘤中心,哈尔滨,150001 |
| 基金项目： | 黑龙江省教育厅科学技术研究项目(12511174) |
| 摘 要： | 目的探讨鼠神经干细胞对鼠胶质瘤C6细胞侵袭能力的影响及机制。方法取新生1～2天SD大鼠大脑皮层组织,于无血清培养基中分离培养神经干细胞并进行NES免疫细胞化学染色,并将神经干细胞和鼠胶质瘤C6细胞于体外共培养,利用2D、3D生长实验和Transwell实验分别检测与鼠神经干细胞共培养鼠胶质瘤C6细胞(实验组)和单独培养鼠胶质瘤C6细胞(对照组)的侵袭能力。结果 2D实验中C6细胞的对照组和实验组的平均直径分别为(18.53±1.32)μm和(13.44±1.45)μm,差异有统计学意义(P<0.01)。3D实验中C6细胞的对照组和实验组的平均直径分别为(20.34±1.25)μm和(15.52±1.43)μm,差异有统计学意义(P<0.01)。Transwell侵袭实验发现胶质瘤C6细胞组中对照组和实验组平均视野侵袭细胞为(36.45±1.36)个和(22.73±1.66)个,差异有统计学意义(P<0.01)。结论与神经干细胞共同培养后C6细胞的侵袭能力明显降低。 |
| 关 键 词： | 胶质瘤 神经干细胞 C6细胞 侵袭 |
Impact on invasiveness of C6 glioma cell by the action of neural stem cells of rat |
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| LIU Nan,YANG Jun-rong,SONG Yue-jia,TONG Zhi-guo,SONG Han-shu,QI Ji-ping. Impact on invasiveness of C6 glioma cell by the action of neural stem cells of rat[J]. Chinese Journal of Clinical and Experimental Pathology, 2011, 27(11) | |
| Authors: | LIU Nan YANG Jun-rong SONG Yue-jia TONG Zhi-guo SONG Han-shu QI Ji-ping |
| Affiliation: | LIU Nan1,YANG Jun-rong1,SONG Yue-jia2,TONG Zhi-guo3,SONG Han-shu1,QI Ji-ping1 (1 Department of Pathology 2Department of Digestive the First Affiliated Hospital of Harbin Medical University,Harbin 150001,China,3Department of Oncosurgery the Fourth Affiliated Hospital of Harbin Medical Unibersity,China) |
| Abstract: | Purpose To explore the impact and mechanism of neural stem cells of rat on the invasiveness of C6 glioma cells of rat.Methods The cerebral cortices of newborn 1-2 day Sprague-Dawley rats(SD rats) were extracted,then the neural stem cells in serumfree medium were separated and cultured.These cells were stained with NSE immunohistochemical staining.After that the neural stem cells were co-cultured with C6 glioma cell of rat in vitro.The invasiveness of neural stem cells of rat which is co-cultured with C6 gli... |
| Keywords: | glioma neural stem cells C6 cells invasion |
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