| 霍山石斛组织培养及快速克隆繁殖技术 |
| |
| 引用本文： | 李蕤,王琳,陈群,樊家荣.霍山石斛组织培养及快速克隆繁殖技术[J].药物生物技术,2011(1):11-15. |
| |
| 作者姓名： | 李蕤 王琳 陈群 樊家荣 |
| |
| 作者单位： | 合肥大学生物与环境工程系; |
| |
| 基金项目： | 安徽省高校自然科学基金资助项目(KJ2009B244Z) |
| |
| 摘 要： | 为药用霍山石斛野生资源的保护和快速克隆繁殖,以霍山石斛幼茎为外植体,利用植物组织培养技术,成功培养了大量的霍山石斛幼苗.研究了培养温度、pH、添加物和激素配比等组织培养条件和调控因素对霍山石斛幼芽增殖的影响.结果表明:在MS为固体培养基,激素组合为O.3 mg/L NAA和O.5 mg/L 6-BA,温度25℃,蔗糖浓...
|
| 关 键 词： | 霍山石斛 外植体 组织培养 快速克隆繁殖 |
Tissue Culture and Rapid Cloning Propagation of Dendrobidium Huoshanness |
| |
| LI Rui,WANG Lin,CHEN Qun,FAN Jia-rong.Tissue Culture and Rapid Cloning Propagation of Dendrobidium Huoshanness[J].Pharmaceutical Biotechnology,2011(1):11-15. |
| |
| Authors: | LI Rui WANG Lin CHEN Qun FAN Jia-rong |
| |
| Institution: | LI Rui,WANG Lin,CHEN Qun,FAN Jia-rong(Department of Biological and Environmental Engineering,Hefei University,Hefei 230022,China) |
| |
| Abstract: | To protect natural resource of medicinal plant Dendrobidium huoshanness and to realize rapid clonig propagation,the chit of Dendrobidium huoshanness was successfully cultured by plant tissue culture techniques using shoot tip of dendrobidium huoshanness as explants.The various conditions of plant tissue culture,such as different additives,temperature,pH value and various hormones ratio for Dendrobidium huoshanness were investigated.The experimental results showed that optimization conditions of plant tissue... |
| |
| Keywords: | Dendrobidium huoshanness Explant Tissue culture Rapid cloning propagation |
| 本文献已被 CNKI 等数据库收录！ |
|
