| 单甲氧基聚乙二醇修饰L-门冬酰胺酶的研究 |
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| 引用本文： | 公伟,薛春佳,冯军,赵文杰. 单甲氧基聚乙二醇修饰L-门冬酰胺酶的研究[J]. 中国医药工业杂志, 2007, 38(5): 336-339 |
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| 作者姓名： | 公伟 薛春佳 冯军 赵文杰 |
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| 作者单位： | 上海医药工业研究院,上海,200040 |
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| 摘 要： | 用一种单甲氧基聚乙二醇修饰剂修饰L-门冬酰胺酶，初步确定了最佳修饰条件，应用阴离子交换色谱和分子筛色谱分离纯化得到单修饰产物，酶活性回收率在40％以上，修饰产物的稳定性提高，免疫原性显著下降。
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| 关 键 词： | L-门冬酰胺酶 单甲氧基聚乙二醇 修饰 纯化 |
| 文章编号： | 1001-8255（2007）05-0336-04 |
| 修稿时间： | 2006-09-08 |
Modification of L-Asparaginase with Monomethoxypolyethylene Glycol |
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| GONG Wei,XUE Chun-jia,FENG Jun,ZHAO Wen-jie. Modification of L-Asparaginase with Monomethoxypolyethylene Glycol[J]. , 2007, 38(5): 336-339 |
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| Authors: | GONG Wei XUE Chun-jia FENG Jun ZHAO Wen-jie |
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| Affiliation: | Shanghai Institute of Pharmaceutical Industry, Shanghai 200040 |
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| Abstract: | A kind of monomethoxypolyethylene glycol was used to modify L-asparaginase and the optimal modification factors were got. The mono-PEGylated enzyme was isolated from the reaction mixture by anion-exchange and gel filtration. The PEGylated enzyme showed better stability, and its activity recovery was maintained above 40% while the antigenicity was greatly reduced. |
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| Keywords: | L-asparaginase monomethoxypolyethylene glycol modification purification |
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