单甲氧基聚乙二醇修饰L-门冬酰胺酶的研究 |
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引用本文: | 公伟,薛春佳,冯军,赵文杰.单甲氧基聚乙二醇修饰L-门冬酰胺酶的研究[J].中国医药工业杂志,2007,38(5):336-339. |
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作者姓名: | 公伟 薛春佳 冯军 赵文杰 |
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作者单位: | 上海医药工业研究院,上海,200040 |
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摘 要: | 用一种单甲氧基聚乙二醇修饰剂修饰L-门冬酰胺酶,初步确定了最佳修饰条件,应用阴离子交换色谱和分子筛色谱分离纯化得到单修饰产物,酶活性回收率在40%以上,修饰产物的稳定性提高,免疫原性显著下降。
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关 键 词: | L-门冬酰胺酶 单甲氧基聚乙二醇 修饰 纯化 |
文章编号: | 1001-8255(2007)05-0336-04 |
修稿时间: | 2006-09-08 |
Modification of L-Asparaginase with Monomethoxypolyethylene Glycol |
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Authors: | GONG Wei XUE Chun-jia FENG Jun ZHAO Wen-jie |
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Institution: | Shanghai Institute of Pharmaceutical Industry, Shanghai 200040 |
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Abstract: | A kind of monomethoxypolyethylene glycol was used to modify L-asparaginase and the optimal modification factors were got. The mono-PEGylated enzyme was isolated from the reaction mixture by anion-exchange and gel filtration. The PEGylated enzyme showed better stability, and its activity recovery was maintained above 40% while the antigenicity was greatly reduced. |
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Keywords: | L-asparaginase monomethoxypolyethylene glycol modification purification |
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