| RP-HPLC法测定蒙药毛勒日-达布斯-4汤中胡椒碱含量 |
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| 引用本文： | 白玉霞,包桂花. RP-HPLC法测定蒙药毛勒日-达布斯-4汤中胡椒碱含量[J]. 辽宁中医杂志, 2007, 34(8): 1132-1133 |
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| 作者姓名： | 白玉霞 包桂花 |
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| 作者单位： | 内蒙古民族大学蒙医药学院,内蒙古,通辽,028041;辽宁中医药大学,辽宁,沈阳,110032 |
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| 摘 要： | 目的:建立蒙药毛勒日-达布斯-4汤中胡椒碱高效液相含量测定方法。方法:使用C18,5μm(150mm×4.6mm)色谱柱;甲醇-水(77∶23)为流动相;流速1.0mL/min;柱温:室温;检测波长:343nm。结果:RP-HPLC分离效果好,线性范围0.0192~0.0959μg。平均加样回收率97.02%,RSD为0.5%。结论:本实验方法简便易行,结果准确可靠,可作为蒙药毛勒日-达布斯-4汤质量控制方法。
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| 关 键 词： | RP-HPLC 蒙药毛勒日-达布斯-4汤 胡椒碱 |
| 文章编号： | 1000-1719（2007）08-1132-02 |
| 修稿时间： | 2007-03-27 |
Determination of Piperine in Mongolian Medicine Maoleri-dabusi-4 Decoction by HPLC Method |
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| BAI Yu-xia,BAO Gui-hua. Determination of Piperine in Mongolian Medicine Maoleri-dabusi-4 Decoction by HPLC Method[J]. Liaoning Journal of Traditional Chinese Medicine, 2007, 34(8): 1132-1133 |
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| Authors: | BAI Yu-xia BAO Gui-hua |
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| Affiliation: | l. College of Mongolian Medicine and Pharmacy of Inner Mongolia University for the Nationalities, Tongliao 028041, Inner Mongolia, China; 2. Liaoning University of TCM , Shenyang 1110032, Liaoning , China |
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| Abstract: | Objective: To establish a HPLC method for determination of piperine in Mongolian medicine Maoleri-dabusi-4 decoction.Methods: HPLC method was carried on C18 5μm(150mm×4.6mm) column using methanol-water(77∶23) as mobile phase.The velocity of flow was 1.0mL/min.Column temperature was room temperature.Detective wavelength was set at 343nm.Results:The linear range of piperine was 0.0192～0.0959μg.The average recovery of piperine was 97.02%,RSD=0.5%.Conclusion:The determination method is simple,accurate and could be applied to the quality control of Mongolian medicine Maoleri-dabusi-4 decoction. |
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| Keywords: | RP-HPLC |
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