| 人精子超低温冻貯技术的研究(三)——不同的甘油浓度、初冻温度和药物对精子存活率的影响 |
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| 引用本文： | 刘金刚,王瑞东,董俊友. 人精子超低温冻貯技术的研究(三)——不同的甘油浓度、初冻温度和药物对精子存活率的影响[J]. 青岛大学医学院学报, 1986, 0(2) |
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| 作者姓名： | 刘金刚 王瑞东 董俊友 |
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| 作者单位： | 青岛医学院附院泌尿外科人类精子库(刘金刚，王瑞东)，青岛医学院附院泌尿外科人类精子库(董俊友) |
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| 摘 要： | 本文对5种不同的甘油浓度、3种不同的初冻温度对冻贮复温后的精子存活率的影响做了比较性研究.首次报道了不同剂量的中药丹参、肌苷对冻贮复温后精子存活率的影响的观察.根据实验结果,作者认为精液中甘油浓度为7%时,冻贮效果最好.
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| 关 键 词： | 精子库 精液保存 人工低温 甘油 肌苷 辅酶A |
STUDIES IN TECHNIC OF CRYOPRESERVATON FOR HUMAN SPERMATOZOA BY LIQUID NITROGEN VAPOR |
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| Liu jingang,et al Section of Human Sperm Bank,Dept. of Urology. STUDIES IN TECHNIC OF CRYOPRESERVATON FOR HUMAN SPERMATOZOA BY LIQUID NITROGEN VAPOR[J]. Acta Academiae Medicinae Qingdao Universitatis, 1986, 0(2) |
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| Authors: | Liu jingang et al Section of Human Sperm Bank Dept. of Urology |
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| Abstract: | This study compared the effects on survival rate of post-thaw sperm of five different glycerol concentrations in mixed semen, three different beginning temperatures of freezing, and first reported the observations on the effects on frozen human sperm of Salvia Miltiorrhiza, Inosinum.The results showed significant differences with the five different glycerol concentrations. Glycerol of 7 percent in mixed semen was the best one. There was no significant difference with the three beginning temperatures of freezing. The drugs mentioned above failed to show beneficial effect on post-thaw sperm. |
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| Keywords: | Sperm bank Semen preservation Induced hypothermia Glycerol Inosine Coenzyme A |
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