改良小口径人工血管的血液相容性研究

目的：研究改良小口径人工血管的血液相容性。方法：将丝素蛋白膜涂覆于聚四氟乙烯小口径人工血管内侧壁，采用等离子体技术对涂覆后的小口径人工血管磺酸化，制成改良小口径人工血管；改良小口径人工血管的溶血相关安全性以体外溶血实验检测，同时行动态凝血、复钙实验评价复合血管血液相容性。结果：溶血试验提示复合血管的溶血率为4．5％，符合材料溶血要求；动态凝血中根据各时间点吸光度平均值可见复合血管组吸光度整体位于普通血管之上，且下降趋势较缓，经历时间较长，与普通血管相比，其抗凝血性能较好；复钙实验中复合血管组平均时间为（450．00±32．18）s，小于对照组平均时间（610．06±37．65）8，两样本t检验提示P=0．000，提示两者差异有统计学意义。结论：改良小口径人工血管具有良好的血液相容性。

The Study of the Modified Small-caliber Artificial Blood Vessel

Objective： To research the hemocompatibility of the modified small-caliber artificial blood vessel （ the combined blood vessel ） . Method： To produce a combined blood vessel with a small-caliber expanded polytetrafluoroethylene vessel coated by silk fibroin film, which sulfonated by low temperature plasma treatment, to evaluated the hemocompatibility and safety of the combined blood vessel including dynamic cruor time test, recalcification time, and the hemolysis test.Result： Hemolytic test indicated that the vascular hemolysis was 4.5%, in line with the requirements of materials hemolytic.The average value of absorbance-clotting time of the combined blood vessel was higher than that of the control blood vessel, and declined more slowly.The recalcification time of the combined blood vessel was significantly longer than that of control blood vessel （ 610.06 ± 37.65 vs 450.00 ± 32.18 seconds, P〈O.01 ） .Conclusion： Form this experiment, we demonstrate the combined blood vessel has a good hemocompatibility.