| 蒽酮-硫酸法测定复方白及乳膏中白及多糖的含量 |
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| 引用本文： | 陶平德,常明泉,李德福,王刚. 蒽酮-硫酸法测定复方白及乳膏中白及多糖的含量[J]. 儿科药学杂志, 2012, 18(9): 40-43 |
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| 作者姓名： | 陶平德 常明泉 李德福 王刚 |
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| 作者单位： | 湖北医药学院附属医院,湖北十堰,442000 |
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| 基金项目： | 太和科研基金资助项目(2009D34);湖北省教育厅优秀中青年基金资助项目(基金编号:Q201002110) |
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| 摘 要： | 目的:建立复方白及乳膏中白及多糖的含量测定方法.方法:以无水葡萄糖为对照品,氯仿-乙醇混合溶液为溶解介质,蒽酮-硫酸法测定,测定波长620 nm.结果:白及多糖在1.9～34.2 μg/mL范围内线性关系良好,A=0.033C-0.0067(γ =0.999 7),回收率为99.55％,RSD=1.12％ (n=6).结论:该方法便捷、重现性好,适用于复方白及乳膏中白及多糖的含量测定.
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| 关 键 词： | 复方白及乳膏 白及多糖 蒽酮-硫酸法 含量测定 |
Content Determination of Bletilla Striata Polysaccharide in Compound Bletilla Striata Cream by Anthrone-Sulfuric Acid Method |
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| Tao Pinde , Chang Mingquan , Li Defu , Wang Gang. Content Determination of Bletilla Striata Polysaccharide in Compound Bletilla Striata Cream by Anthrone-Sulfuric Acid Method[J]. Journal of Pediatric Pharmacy, 2012, 18(9): 40-43 |
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| Authors: | Tao Pinde Chang Mingquan Li Defu Wang Gang |
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| Affiliation: | (Taihe Hospital of Hubei Medical College,Hubei Shiyan 442000,China) |
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| Abstract: | Objective: To establish the quantitative determination method of the bletilla striata polysaccharide in Compound Bletilla Striata Cream.Methods: Using the anhydrous dextrose as the control,the bletilla striata polysaccharide was dissolved in the mixture of chloroform and alcohol.Anthrone-Sulfuric acid method was used and the wavelength was 620 nm.Results: The bletilla striata polysaccharide curve was linear within a range of 1.9~34.2 μg/mL.Linear equation was A=0.033C-0.0067(r=0.999 7).The average recovery was 99.55% with RSD of 1.12%(n=6).Conclusions: This method is convenient,stable,and can be used to assay content of the bletilla striata polysaccharide in Compound Bletilla Striata Cream. |
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| Keywords: | Compond bletilla striata cream Bletilla striata polysaccharide Anthrone-sulfuric acid method Determination |
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