电针对实验性家兔视网膜神经递质的影响

目的:研究电针对家兔视网膜中几种神经递质的影响,深入探讨针刺影响视觉功能的机制。方法:电针家兔合谷穴及太溪穴15min后,立即处死,低温下取出视网膜,采用氨基酸自动分析,测定视网膜中谷氨酸、甘氨酸、天门冬氨酸。采用Ellman改良法测定胆碱脂酶。结果:针刺太溪穴可以使L—谷氨酸的释放增加,针刺合谷穴释放减少,两者比较有极显著性差异(P<0.01),太溪穴与正常组比较有显著性差异(P<0.05);针刺太溪可以使天门冬氨酸的释放增加,针刺合谷使释放减少,两者间有极显著性差异(P<0.01),但分别与正常比较无差异(P<0.05)。甘氨酸和胆碱酯酶活性的变化各组间变化不大。结论:针刺可以引起视网膜神经递质的变化,同时这种针刺效应具有相对的穴位特异性。

Effects of electric acupuncture on the neurotransmitters of rabbit retina

AIM: To innvestigate the effects and mechanisms of electric acupuncture on the neurotransmitters of the rabbit retina and the visual function. METHODS:The Hegu point and Taixi point were stimulated for 15min, animals were sacrificed immediately and the retina were isolated under low temperature. The Glu, Gly, r-GABA of the retina were analysed by amino acid analysator and the cholinesterase were determined by Ellman method. RESULTS: The L-Glu increased after stimulating the Taixi point, while the L-Gly decreased while sitmulating the Hegu point, the difference between them was very remarkable (P<0.01). When comparing the group of Taixi point with the normal group, the difference was significant (P<0.05). Stimulating the Taixi point increased the Asp releasing, while stimulating the Hegu point decreased the Asp, the difference was remarkable (P<0.01). However, when comparing them separately with the normal group, the differences was not remarkable (P>0.05). The levels of Gly and cholinesterase changed slightly in each group. CONCLUSION: Electric acupuncture can induce the change of neurotransmitters in the rabbit retina, the effects are point specified.