微血管密度在中耳胆脂瘤上皮增殖中的意义

目的　研究微血管密度在中耳继发性胆脂瘤上皮增殖演变过程中的意义。方法　应用免疫组化ABC染色方法和计算机图像分析系统,连续观察 15例具有典型鼓膜后皱襞处穿孔的中耳胆脂瘤患者的不同部位(鼓膜穿孔部位邻近皮肤和耳道深部正常皮肤 )中血管参数的改变,并和20例非胆脂瘤型中耳炎鼓膜穿孔部位邻近皮肤作对比。结果　胆脂瘤型中耳炎患者的鼓膜穿孔部位邻近皮肤的微血管计数、微血管相对面积 ( x±s)分别为 14.395±2.000和 (9.927±2.600 ) %,显著高于其自身耳道深部正常皮肤的 6 218±0 949和 ( 5.076±0.807 )% (P<0. 001 );亦显著高于非胆脂瘤型中耳炎鼓膜穿孔部位邻近皮肤的 6.163±1 051和 ( 5.785±1.428 )% (P<0.001 )。结论中耳胆脂瘤的鼓膜穿孔部位邻近皮肤增生活跃。

Role of microvessel density on human middle ear cholesteatoma

OBJECTIVE: To explore the role of microvessel density in acquired middle ear cholesteatoma, to analyze it playing the possible role in proliferative ability of cholesteatoma epidermis. METHODS: The specimens from 15 cases acquired middle ear cholesteatoma patients having typical perforation in the posterior fold (the adjacent skin around perforation and the normal deep external ear skin) were examined by immunohistochemical ABC method. The microvessel density was analyzed by computer, and was compared with 20 cases non-cholesteatoma otitis media's the adjacent skin around perforation. RESULTS: Counts of microvessel and relative area of all microvessels per image in the adjacent skin around perforation were 14.395 +/- 2.000 and (9.927 +/- 2.600)%, respectively. These were significantly higher than the normal deep external ear skin's with the average of 6.218 +/- 0.949 and (5.076 +/- 0.807)% in 15 cases acquired middle ear cholesteatoma patients (P < 0.001). The adjacent skin around perforation of cholesteatoma was also significantly higher than non-cholesteatoma's with the average of 6.163 +/- 1.051 and (5.785 +/- 1.428)% (P < 0.001). CONCLUSIONS: The adjacent skin around perforation in human middle ear cholesteatoma had a relatively hyperproliferative ability.