HPLC-ELSD法测定银杏叶茶中总内酯的含量

目的：采用RP-HPLC法测定低酚酸银杏叶保健茶中总内酯的含量。方法：采用AgilentC18（4.6mm×250mm,5μm）色谱柱,以甲醇-四氢呋喃-水（25：10：65）为流动相,柱温为30℃,蒸发光散射检测器检测。结果：银杏叶保健茶中银杏内酯A、银杏内酯B、银叶内酯C和白果内酯的进样量的对数与峰面积的对数线性关系较好,线性范围分别为0.485～9.701μg、0.460～9.204μg、0.501～11.899μg、0.935～18.703μg;银杏内酯A、银杏内酯B、银叶内酯C和白果内酯加样回收率分别为100.45%（RSD为2.79%）、98.29%（RSD为2.90%）、98.22%（RSD为2.53%）、101.32%（RSD为1.81%）。结论：本方法简便、准确、分离效果好,适合于银杏叶茶总内酯的含量测定。

Determination of Ginkgolides in teas of Ginkgo biloba by HPLC-ELSD

Objective：To establish an RP-HPLC method for quantitative determination of Ginkgolides in teas of Ginkgo biloba.Methods：The evaporation light scattering detector（ELSD） were employed.The analytical column was C18 column（4.6 mm× 250 mm,5 μm）.The mobile phase was composed of methanol-tetrahydrofuran-water（25：10：65） at a flow rate of 1.0 ml/min.Results：The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of ginkgolide A,B,C and bilobalide was 0.485～9.701 μg,0.460～9.204 μg,0.501～11.899 μg,0.935～18.703 μg respectively.The average recovery was 100.45%（RSD=2.79%）,98.29%（RSD=2.90%）,98.22%（RSD=2.53%）,101.32%（RSD=1.81%） respectively.Conclusion：This method is sensitive,repeatable and suitable to determine the content of total Ginkgolides in teas of Ginkgo biloba.