香烟烟对血管内皮表达细胞间粘附分子-1的影响

目的探讨香烟烟对人血管内皮表达细胞间粘附分子-1的影响。方法以香烟烟为抗原刺激巨噬细胞株细胞，再用其上清液刺激人脐带静脉内皮细胞。采用ELISA法分别测定了经抗原刺激后3h、6h、12h、24h的巨噬细胞上清液中的肿瘤坏死因子-α(TNF-α)；采用同样方法分析用抗原刺激巨噬细胞株细胞的上清处理血管内皮细胞3h、6h、9h、12h、24h培养基中可溶性细胞间粘附分子-1(sICAM-1)。结果随着处理时间的延长，TNF-α的表达量增多，实验组(4.967±0.802)pg/ml较对照组(3.181±0.214)pg/ml含量明显增加。统计结果提示，随孵育时间延长，sICAM-1表达的量增加，并且观察组(均数为0.0684ng/ml)比对照组(均数0.0459ng/ml)增加49%。结论香烟烟可以刺激人体最主要的抗原递呈细胞-巨噬细胞表达TNF-α，进一步使血管内皮细胞表达ICAM-1增加。

Effects of tobacco smoke on the expression of ICAM-1 in human umbilical vein endothelial cells

OBJECTIVE: To study the effect of tobacco smoke on the expression of intercellular adhesion molecule (ICAM)-1 in endothelial cell. METHODS: Macrophages were challenged in vitro with cigarette smog, and the supernatant was collected at 3 h, 6 h, 12 h and 24 h after tobacco treatment. The tumor necrosis factor-alpha (TNF-alpha) was measured by the ELISA method. Then the human umbilical vein endothelial cells (HUVEC) were incubated with the supernatant from the macrophages stimulated by cigarette smoke allergen. ICAM-1 in the supernatant was detected by the ELISA method. RESULTS: TNF-alpha was increased with the longer incubation time. A significant difference of TNF-alpha(t = -4.301, P < 0.01) was observed between the tobacco treated group (4.967 +/- 0.802 pg/ml) and the control group (3.181 +/- 0.214 pg/ml). Soluble ICAM-1 in HUVEC in the tobacco treated group (mean 0.068 4 ng/ml) increased 49% than the controls (mean 0.045 9 ng/ml). CONCLUSION: The results suggested that cigarette smog enhances the expression of TNF-alpha in the antigen-presenting cell(APC)-macrophage, and the latter up-regulates the secretion of the membrane glycoprotein sICAM-1 in HUVEC which can assist leukocyte immigration.