| 次声对PHA和同种异体抗原诱导小鼠脾细胞增殖的影响 |
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| 引用本文： | 杨琨,张建平,金伯泉. 次声对PHA和同种异体抗原诱导小鼠脾细胞增殖的影响[J]. 细胞与分子免疫学杂志, 2000, 16(4): 289-291 |
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| 作者姓名： | 杨琨 张建平 金伯泉 |
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| 作者单位： | 第四军医大学基础部免疫学教研室!陕西西安710032(杨琨，张建平，金伯泉)，第四军医大学西京医院理疗科!陕西西安710032(陈景藻) |
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| 基金项目： | 全军医药卫生科研基金重点资助!No.96Z042 |
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| 摘 要： | 目的观察次声对PHA诱导小鼠脾细胞增殖及同种异体抗原诱导T细胞增殖反应的影响。方法应用次声压力舱 ,建立小鼠动物实验模型。以8Hz、120dB次声作用于8wk龄雌性Balb/c鼠 ,每天作用2h ,连续作用14d。取小鼠脾细胞 ,用PHA或同种异体抗原刺激后 ,采用3H TdR掺入法检测脾细胞的增殖情况。结果次声能显著促进PHA诱导的小鼠脾细胞增殖 ,刺激指数(SI)为4.73±0.17 ,与正常对照组的SI(2.12±0.05)相比较 ,提高了2.23倍(P<0.01)。而次声对同种异体抗原诱导的T细胞增殖具有明显的下调作用 ,SI为2.48±0.12 ,较正常对照组4.59±0.32 ,降低45.97 %(P<0.01)。结论次声能够影响机体免疫淋巴细胞的功能。
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| 关 键 词： | 次声 PHA 同种异体抗原 脾细胞 增殖 |
| 修稿时间： | 2000-01-06 |
The effect of infrasound on the proliferation of splenocytes induced by PHA and alloantigen |
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| YANG Kun,ZHANG Jian ping,JIN Bo quan,CHEN Jing zao. The effect of infrasound on the proliferation of splenocytes induced by PHA and alloantigen[J]. Chinese journal of cellular and molecular immunology, 2000, 16(4): 289-291 |
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| Authors: | YANG Kun ZHANG Jian ping JIN Bo quan CHEN Jing zao |
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| Affiliation: | YANG Kun1,ZHANG Jian ping1,JIN Bo quan1,CHEN Jing zao21Department of Immunology,Faculty of Preclinical Medicine,2Department of Physiotherapy,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China |
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| Abstract: | Aim To investigate the effect of infrasound on the proliferation of splenocytes induced by PHA and alloantigen. Methods Balb/c mice were exposed to infrasound of 8 Hz with the intensity of 120 dB (2 hours per day for 14 consecutive days). Then 3H TdR incorporation assay was employed to detect the proliferation of splenocytes induced by PHA and alloantigen. Results The level of PHA stimulated proliferation of splenocytes was marke dly high in infrasound group(P<0.01),but the alloantigen indu ced proliferation of splenocytes in infrasound group was lower than that in control group(P<0.01). Conclusion Infrasound can influence the function of immune lymphocytes in body. |
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| Keywords: | infrasound PHA alloantigen splenocyte proliferation |
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