经冠状动脉移植自体BM-MNCs治疗猪急性心肌梗死的作用

目的探讨冠状动脉内移植自体骨髓单个核细胞（BM—MNCs）治疗猪急性心肌梗死的有效性。方法实验动物分为冠脉内移植MNCs组（n=6）及冠脉对照组（n=5）。冠脉内移植BM—MNCs后4周观察移植细胞归巢情况、小血管密度、心功能变化。结果冠脉内MNCs移植后4周，心肌细胞间可见发蓝色荧光的移植细胞，散在分布，心肌细胞间有较多新生毛细血管并可见新生的心肌样细胞。移植组小血管密度、左室射血分数（LVEF）及短轴缩短率（FS）明显高于对照组（P〈0．01），左室舒张末期内径（LVEDd）低于对照组（P〈0．05）。结论经冠脉移植的BM—MNCs可归巢到宿主心肌，有促进缺血心肌血管新生、改善左室收缩功能、减轻心室重构的作用。

Experimental study of autologous BM-MNCs by intracoronary transplantation into ischemic myocardium of AMI in swines

Objective To test the effectiveness of BM-MNCs by intracoronary transplantation. Methods 11 swines were divided into two groups： BM-MNCs group （n=6） and confrol group （n=5）. Four weeks after implantation, BM-MNCs homing, blood vessels density, heart function were observed. Results Four weeks after implantation, blue fluorescence cells can be detected in host myocardium. Many newborn capillary vessels and a few cardiomyocytes-like were observed. Blood vessels density and LVEF and FS in BM-MNCs group were significantly higher than those in control group （P〈0.01） and LVEDd were lower than those in control group （P〈0.05）. Conclusion Intracoronary transplantation BM-MNCs are capable of homing to host myocardium, which can induce angiogenesis and improve cardiac function and attenuate remodeling of the left ventricle.