高糖刺激人肾脏成纤维细胞肝细胞生长因子和c-met的表达及其意义

目的 在体外高糖刺激人肾脏间质成纤维细胞时观察其肝细胞生长因子（HGF）和c-met的表达。方法 将人肾脏间质成纤维细胞分别培养于不同浓度的葡萄糖中，于24h后用RT－PCR方法检测HGF、c-met和1型纤溶酶原激活物抑制物（PAI－1）mRNA的转录水平，用不同浓度的HGF刺激细胞来观察PAI－1mRNA的表达。结果 高浓度葡萄糖培养的细胞早期就有HGF、c-met表达，之后表达逐渐下降，而PAI－1在此之后出现表达上升。同时外源性HGF可以抑制PAI－1mRNA的表达。结论 HGF在肾脏中是潜在的一种抗纤维化的细胞因子，可以通过降低PAI－1的表达，从而促进细胞外基质的降解。

Expression of hepatocyte growth factor and c-met stimulated by high glucose in human kidney fibroblast and its significance

Objective To observe high glucose induced expression of hepatocyte growth factor (HGF) and c met in human kidney fibroblast. Methods The effects of glucose concentrations on expression of HGF, c met and plasminogen activator inhibitor (PAI) 1 in cultured human kidney fibroblasts were observed by RT PCR. In the same system, the effect of exogenous HGF on the expression of PAI 1 was investigated. Results Human kidney fibroblasts cultured in high glucose concentration (25 mmol/L) showed higher HGF and c met expressions in the early stage and then manifested a gradient decrease of HGF and c met expressions, but PAI 1 expression was gradiently increased. Exogenous HGF resulted in inhibiting PAI 1 expression. Conclusion HGF is a potential anti fibrogenic factor and activates matrix degradation pathways in diabetic kidney by reducing PAI 1 expression.