血小板生成素和转化生长因子-β_1在儿童血液病中的研究

目的探讨血液病儿童血清和骨髓上清液中TGF-β1、TPO的水平及其临床意义。方法采用ELISA法测定急性特发性血小板减少性紫癜、再生障碍性贫血、急性淋巴细胞白血病患儿血清及骨髓上清液中TGF-β1、TPO水平。结果ITP患儿血清TPO水平高于对照组,但差异无显著性(P>0.05),TGF-β1水平明显高于对照组(P<0.01),骨髓上清液中TPO和TGF-β1水平均高于对照组(P均<0.01);AA患儿血清和骨髓TPO水平显著增高,与健康对照组比较具有显著性差异(P<0.01),ALL患儿TPO水平虽低于AA组,但仍明显高于对照组(P<0.01);AA组血清TGF-β1水平与正常对照组比较无显著性差异,ALL患儿血清及骨髓上清液中TGF-β1水平较对照组显著降低(P<0.01),CR后血清中和骨髓中TGF-β1水平上升,CR前后TGF-β1水平比较具有显著性差异(P<0.01)。结论TGF-β1、TPO在儿童血液病的病理生理过程中发挥重要作用,动态检测TGF-β1水平可为ALL患儿病情评估、预后估计提供客观依据。

A study on thrombopoietin and transforming growth factor -beta1 in children with hematological diseases

Objetive To study the prognostic significance of thrombopoietin(TPO),transforming growth factor beta 1(ΓGF-β_1) in children with hematological diseases.Methods TGF-β_1 and TPO levels in the serum and bone marrow of children with ITP,AA,ALL were detected using enzyme-linked immunosorbent assay(ELISA).Results The serum level of TPO in ITP children was higher than controls,but had no significant difference.The serum level of TGF-β_1 in ITP children was much higher than controls(P<0.01).The bone marrow level of TPO and TGF-β_1 in ITP children was higher than controls(P<0.01).In AA children,the serum was higher than in normal and had significant difference(P<0.01).In ALL children, the serum TPO was lower than in AA children but much higher than in normal(P<0.01).In AA children,the serum TGF-β_1 was higher than in normal with no significant difference(P>0.05) In ALL children,the serum TGF-β_1 was lower than in normal(P<0.01).The level of TGF-β_1 in the serum and bone marrow of children with ALL would increase to nearly the normal level when remission occurred.The level of TGF-β_1 after therapy was higher than before treatment and control(P<0.01).Conclusion Dynamic changes in the serum and bone marrow of TGF-β_1 and TPO can be used as an indicator of therapeutic effect as well as prognosis in children with hematological diseases.